|product name||Proteinase K|
|place of origin||Beijing|
Storage Temperature:Stored at room temperature for three years.
Proteinase K is a non-specific serine protease, and the RNase and DNase activities have been removed after purification.Proteinase K is active in the presence of SDS or urea as well as in a wide range of pH, salt concentration and temperature, and can be increased in 1% SDS.Proteinase K is widely used in the preparation of pulsed electrophoresis chromosomal DNA, western blot and genomic DNA. The working concentration is usually 50-500g/mL, and the specific working concentration is determined according to whether the buffer solution contains SDS, urea, pH, temperature and other factors.
1. RNase and DNase are not included;
2. Active in denaturant, such as urea and SDS;
3. Active in metal chelators, such as EDTA;
4. Active in a wide pH range (pH 4-12.5), the best pH range is pH 7.5-8.0;
5. Active in a wide range of temperature, generally operating temperature range of 50℃-65℃;
6. ddH2O can be dissolved;
7.Enzyme activity value: ≥30 U/mg Lyophilized powder.
Method of inactivation:
After heating at 95℃-98℃ for 10 min, or adding PMSF or DFP inhibitor into the reaction system, it was deactivated.
1. The general storage concentration is 10-20 mg/ml to avoid repeated freeze-thaw;
2.Ca2+ can increase the thermal stability of protease K.
Beijing bomeide Gene Technology Co., Ltd. is a professional enterprise of DNA sequencing, DNA synthesis, molecular biological reagents and technical services. The company not only has a rigorous and efficient R & D, production team, but also has a professional sales team and technical service team to provide you with efficient and efficient professional services.
Beijing bomeide Gene Technology Co., Ltd. is a professional enterprise of DNA sequencing, DNA synthesis, molecular biological reagents and technical services.