Limited time sale Proteinase K Lyophilized Powder

Share on:


Price:3 200,00 $
Price in USD:Details

Quantity:


Product Overview

Description


3-(1)_01.jpg89.jpg_20201009211239.jpg

















product nameProteinase K
 modelSH458-100
 place of originBeijing

Storage Temperature:Stored at room temperature for three years.


 


 Product Introduction:


Proteinase K is a non-specific serine protease, and the RNase and DNase activities have been removed after purification.Proteinase K is active in the presence of SDS or urea as well as in a wide range of pH, salt concentration and temperature, and can be increased in 1% SDS.Proteinase K is widely used in the preparation of pulsed electrophoresis chromosomal DNA, western blot and genomic DNA. The working concentration is usually 50-500g/mL, and the specific working concentration is determined according to whether the buffer solution contains SDS, urea, pH, temperature and other factors.


 


 Product nature:


1. RNase and DNase are not included;


2. Active in denaturant, such as urea and SDS;


3. Active in metal chelators, such as EDTA;


4. Active in a wide pH range (pH 4-12.5), the best pH range is pH 7.5-8.0;


5. Active in a wide range of temperature, generally operating temperature range of 50℃-65℃;


6. ddH2O can be dissolved;


7.Enzyme activity value: ≥30 U/mg Lyophilized powder.


 


 Method of inactivation:


After heating at 95℃-98℃ for 10 min, or adding PMSF or DFP inhibitor into the reaction system, it was deactivated.


 


 Remark:


1. The general storage concentration is 10-20 mg/ml to avoid repeated freeze-thaw;


2.Ca2+ can increase the thermal stability of protease K.


3-(1)_03.jpg


    Beijing bomeide Gene Technology Co., Ltd. is a professional enterprise of DNA sequencing, DNA synthesis, molecular biological reagents and technical services. The company not only has a rigorous and efficient R & D, production team, but also has a professional sales team and technical service team to provide you with efficient and efficient professional services.


3-(1)_05.jpg3-(1)_07.jpg


88_07.jpg


Beijing bomeide Gene Technology Co., Ltd. is a professional enterprise of DNA sequencing, DNA synthesis, molecular biological reagents and technical services.


4_.jpg


0.0099 s.