Горячая продажа домашняя чистая протеиназу K порошок

Product Overview

Description

Storage Temperature:Stored at room temperature for three years.

 Product Introduction:

Proteinase K is a non-specific serine protease, and the RNase and DNase activities have been removed after purification.Proteinase K is active in the presence of SDS or urea as well as in a wide range of pH, salt concentration and temperature, and can be increased in 1% SDS.Proteinase K is widely used in the preparation of pulsed electrophoresis chromosomal DNA, western blot and genomic DNA. The working concentration is usually 50-500g/mL, and the specific working concentration is determined according to whether the buffer solution contains SDS, urea, pH, temperature and other factors.

 Product nature:

1. RNase and DNase are not included;

2. Active in denaturant, such as urea and SDS;

3. Active in metal chelators, such as EDTA;

4. Active in a wide pH range (pH 4-12.5), the best pH range is pH 7.5-8.0;

5. Active in a wide range of temperature, generally operating temperature range of 50℃-65℃;

6. ddH2O can be dissolved;

7.Enzyme activity value: ≥30 U/mg Lyophilized powder.

 Method of inactivation:

After heating at 95℃-98℃ for 10 min, or adding PMSF or DFP inhibitor into the reaction system, it was deactivated.

 Remark:

1. The general storage concentration is 10-20 mg/ml to avoid repeated freeze-thaw;

2.Ca2+ can increase the thermal stability of protease K.

    Beijing bomeide Gene Technology Co., Ltd. is a professional enterprise of DNA sequencing, DNA synthesis, molecular biological reagents and technical services. The company not only has a rigorous and efficient R & D, production team, but also has a professional sales team and technical service team to provide you with efficient and efficient professional services.

Beijing bomeide Gene Technology Co., Ltd. is a professional enterprise of DNA sequencing, DNA synthesis, molecular biological reagents and technical services.