Набор для прямого ПЦР-анализа листьев растений

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Price:150,00 $
Price in USD:Details

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Product Overview

Description


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Description:


 


This product uses a unique lysis buffer system to lyse plant leaves. The lysate can be used as a template without purification. After adding the primers, the PCR Mix of this kit can be used for amplification.


 


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Specifications:


 


50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns


 


Kit components:


 























Part I



Buffer PS1



Buffer P2



DNA Loading Buffer



Part II



Buffer PS2



Leaf PCR EasyTM Mix



 


 


Features&advantages:


 


-No time-consuming and expensive DNA purification


-Less material


-Simple—the sample can be subjected to PCR reaction or lysis reaction without cutting or grinding


-2 X Mix—reducing sample loading errors and reaction system preparation time


-Fast—template preparation can be completed in 10 minutes, PCR reaction can be completed in 50 minutes


-High throughput—lysis reaction can be completed in 96-well PCR plate


 


Kit parameters:


 


-Application: Identification of transgenic plants, genotyping, etc.


-Sample: plant leaves rich in polysaccharide or polyphenol


-Sample size: Diameter 2-3mm (direct method), diameter 5-7mm (lysis method)


-Detection range: PCR product ≤1kb


   


Work Flow: 


 


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Electropherogram:


 


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Storage and Shelf life:


 


Part I of this kit should be stored at room temperature or 2-8℃.


-Reagents Buffer PS1, Buffer P2, 6×DNA Loading Buffer can be stored for 12 months under dry conditions; if needed to be stored for a longer time, the reagents can be stored at 2-8℃.


Part II of this kit should be stored at -20℃.


-Reagents Buffer PS2, 2×Leaf PCR EasyTM Mix, if used frequently, can also be stored at 4℃ for short-term storage (use up within 10 days).


 


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   Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the worlds leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.


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   Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregenes R&D team consists of 40 people(mainly phD and master), has 2500  comprehensive building, 600  P2 laboratory, and 1200 GMP production facility.


With Direct PCR technology, theres no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregenes Direct PCR technology meet the needs of clinical diagnosis.


 


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