Foregene Factory Supply Mouse Tail Direct PCR Kit(65 centigrade lysis)

Product Overview

Description

 

 

 

 

 

 

 

Foregene Factory Supply Mouse Tail Direct PCR Kit(65  centigrade lysis)

 

Description:

 

This kit uses a unique lysis buffer system to quickly release genomic DNA from mouse tails, ears, muscles and other tissue samples for PCR reactions, so it is particularly suitable for large-scale genetic testing.

The process of releasing genomic DNA from the lysis buffer is completed within 10-30 minutes at 65°C. No other processes such as protein and RNA removal are required, and the released trace DNA can be used as a template for PCR reaction.

2× M1-PCR Easy^TM Mix (UNG) uses dUTP instead of dTTP on the basis of 2× M1-PCR Easy^TM Mix (UNG), and adds UNG enzyme (Uracil-N-glycosylase) that can degrade the template containing dUTP at the same time. Before the PCR reaction, the UNG enzyme is used to degrade the PCR product containing uracil. The UNG enzyme will not have any effect on the template that does not contain uracil, thereby ensuring the specificity and accuracy of amplification and preventing the possibility of large-scale genetic testing. Contamination of PCR products occurred.

D-Taq DNA polymerase is a DNA polymerase specially developed by Foregene for direct PCR reactions. D-Taq DNA polymerase has strong tolerance to a variety of PCR reaction inhibitors, and can efficiently amplify trace amounts of DNA in various complex reaction systems, and the amplification speed can reach 2Kb/min, which is especially suitable for Direct PCR reaction.

 

 

Specifications:

 

50×20µl rxns,200×20µl rxns,500×20µl rxns,2000×20µl rxns

 

Kit components:

 

Part I	Buffer MP
	Foregene Protease Plus
	6× DNA Loading Buffer
Part II	2× M1 PCR EasyTM Mix(UNG)
Instructions

 

 

Features&advantages:

 

-No time-consuming and expensive DNA purification is required.

-The sample demand is small, as little as 5mg mouse tissue or 2-5mm mouse tail can be tested.

-No special treatments such as grinding and crushing are required, and the operation is simple.

-The optimized PCR system makes PCR have higher specificity and stronger tolerance to PCR reaction inhibitors.

-Anti-pollution PCR system 2×M1-PCR Easy^TM Mix (UNG), which effectively eliminates the pollution caused by PCR products and ensures the specificity and accuracy of amplification.

 

Kit parameters:

 

Scope of application: mouse tail, mouse ear, muscle and other tissues.

DNA released from sample lysis: only used as a PCR template.

The kit can be used for the following purposes: identification of transgenosis, animal genotyping, etc.

 

Work Flow: 

 

 

 

 

 

 

Storage and Shelf life:

 

Part I of this kit should be stored at 2-8℃.

The reagent Buffer AL can be stored for 12 months under dry conditions; it can be stored at 2-8℃  for longer storage.

-Reagent Foregene Protease has a unique formula. To ensure its activity and stability, please store it at 4℃ for 12 months.

-Reagent 6×DNA Loading Buffer can be stored at 4℃ or -20℃ for a long time.

Part II of this kit should be stored at -20℃ .

-Reagent 2×M1 PCR Easy^TM Mix(UNG), if is used frequently, it can also be stored at 4℃ for short-term storage (use up within 10 days).

 

 

 

   Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.

With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.

 

 

  Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.