5T free sample Stool DNA Isolation Kit for genomic DNA purification

Product Overview

Description

 

 

Description:

 

This kit provides a quick and easy method to extract genomic DNA from bacteria and food residues from stool samples from various sources. There are a large number of inhibitors in stool samples. Even if these substances are present in a small amount in the purified DNA, they will affect downstream reactions, such as PCR and restriction enzyme digestion. Therefore, the key to purification of fecal DNA is how to effectively remove inhibitors in feces.

This kit uses DNA-Only Column that can specifically bind DNA, a brand-new Foregene Protease and a unique buffer system, which can effectively remove various inhibitors in feces, without organic solvent extraction or ethanol and isopropanol precipitation. The DNA extraction operation from stool samples can be completed in 40 minutes.

 

 

Kit components:

 

Stool DNA Isolation Kit
Kit contents	DE-05711	DE-05712	DE-05713	DE-05714	DE-05715
	5 T	25 T	50 T	100T	250T
Buffer SL1	3.5ml	18ml	35ml	70ml	175ml
Buffer SL2	3.5ml	18ml	35ml	70ml	175ml
Buffer SL3	3.5ml	18ml	35ml	70ml	175ml
Buffer PW	3ml	15ml	30ml	60ml	150ml
Buffer WB	2.5ml	12.5ml	25ml	50ml	125ml
Buffer EB	1.5ml	6ml	15ml	25ml	75ml
Buffer TE	6ml	30ml	60ml	120ml	300ml
Foregene Protease	280μl	1.4ml	1.4mlx2	5.6ml	14ml
Lysozyme	50mg	250mg	500mg	500mgx2	500mgx5
DNA-Only Column	5 sets	25 sets	50 sets	100 sets	250 sets
IFU	1 piece	1 piece	1 piece	1 piece	1 piece

 

Features&advantages:

 

-No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove the RNA from the genomic DNA without adding RNase during the experiment, preventing the laboratory from being contaminated by exogenous RNase.

-Fast speed: The operation is simple, and the fecal genomic DNA extraction operation can be completed within 40 minutes.

-Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

-Safety: No organic reagent extraction is required.

-High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Kit parameters:

 

Kit Application:

It is suitable for purification of genomic DNA from the following samples: fresh or frozen human, cow, chicken, mouse and other fecal samples.

 

Genomic DNA Appliaction:

The genomic DNA purified by Stool DNA Isolation Kit has high purity and can be used for routine molecular biology operations, such as: restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.

 

Work Flow:

Storage and Shelf life:

-This kit can be stored for 12 months under dry conditions at room temperature (15-25°C); if it needs to be stored for a longer period of time, it can be stored at 2-8°C.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

-Foregene Protease solution has a unique formula, which is active when stored at room temperature for a long time (3 months), its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20°C.

-Dry Lysozyme powder is stored at -20°C, the prepared Lysozyme solution is divided into small portions and stored at -20°C.

 

 

Our Services

1. Sample require: we can provide sample.

    More details, Contact me by the following please.   

    Name: Maggie Ma

    Whats app: +8615281067355

    We Chat: +8615281067355

    QQ: 694285120

2. Quick delivery, usually within 1 to 3 days after your payment, within 10 days with mass quantity.

3. Fast and safe Shipping methods, such as  FEDEX, TNT, UPS, EMS.

4. Various and convenient payment methods available, such as T/T, Western Union.

5. We insist: Customer first, high quality, best service for each customers.

 

Company Introduction

  Foregene Co., Ltd. is specialized in the molecular biology lab reagents, IVD raw materials, IVD Kits, and related products and services.Foregene is integrated in R&D, manufacturing and marketing together .

 

   Established in 2011, Foregene has owned the world-class Direct PCR Platform,DNA-Only,RNA double spin column Isolation Platform. The main products include Direct PCR reagents, DNA/RNA isolation kits, PCR Master Mix, Genotyping kits, FISH Probe Kits, IVD Raw Materials, various IVD kits, and Lab Equipments. Foregene can provide the whole solution of IVD products based on our technology Platforms.

 

   Becuse of the world-class leading technology, we are popular in domestic and overseas markets.Foregene’s high quality products are widely used in hospitals, universities, national research institutions, medical laboratories,CRO companies, bio-pharm companies, etc.

 

  Looking forward to Cooperate with you.