Professional Trader of Anti ABDAB Blood Grouping Reagent/ Diagnosis Test Kit Set Available with Private Label

Professional Trader of Anti ABDAB Blood Grouping Reagent/ Diagnosis Test Kit Set Available with Private Label

Intended Use:
Diagnostic kit of rapid slide & modified tube test for qualitative detection of ABO blood group antigens on human red blood cells by immunoagglutination method.

INTRODUCTION:
The mouse monoclonal IgM Anti-A, Anti-B and Anti-AB antibodies are produced “in-vitro” as culture supernatent of selected hybridoma obtained the fusion of mouse antibody producing B-lymphocytes with mouse myelomacells. MONOCLONAL SERA ANTI-A, ANTI-B and ANTI-AB are specific IgM immunoglobulin which are directed against the human red blood cell antigens A,B and AB respectively. MONOCLONAL SERA immunoglobulins are produced from individual cell line hence they are identical in their chemical structure. The Rho (D) antigen is found on erythrocytes of approximately 95% of the Indian population. The terms “Rh” positive or “Rh” negative are understood to refer solely to the presence or absence of this antigen accordingly. Anti D (Rho) monoclonal IgM is used for the detection of the presence of Rho antigen on the red blood cells.

BASIC PRINCIPLE

Human red cells possessing A and/or B blood group antigen will be agglutinated by MONOCLONAL SERA directed towards the respective antigen (s), indicating positive test. Absence of agglutination cell with Anti-A, Anti-B and Anti-AB reagents is a negative test results and indicates the absence of the corresponding antigen. Human red cells possessing RhoD antigen will be agglutinated by MONOCLONAL SERA directed towards the respective antigen (s), indicating positive test. Absence of agglutination cells with ANTI-D (IgM), ANTI-D (IgG + IgM), Anti - D (IgG),Anti D (Rho IgG + IgM) reagents is a negative test results and indicates the absence of the corresponding antigen.

SAMPLE COLLECTION & STORAGE
Whole blood with anticoagulant. Incase of delay in testing store sample at 2-8°C.

Working reagent preparation and stability

• Store the reagent at 2-8°C. DO NOT FREEZE.


• Unopened vial of MONOCLONAL SERA are stable at 2-8°C till the opened vial within a month.

TITRE & AVERAGE AVIDITY

1:512 for Anti A and Anti B. 1:256 for Anti D (IgG+IgM) Macroscopically & Average avidity <10 seconds with whole blood.

PROCEDURE
I. SLIDE TEST:
1. Place one drop of MONOCLONAL SERA Anti-A, Anti-B, Anti-D and Anti-AB on clean and dry slide.
2. To each drop of reagent, add one drop of whole blood mix well with an applicator stick.
3. Rock the slide gently back and forth.
4. Observe for agglutination macroscopically within 2 minutes.

II. TUBE TEST:

1. Prepare 5% suspension of the RBCs to be tested in isotonic saline.
2. Place one drop of MONOCLONAL SERA Anti-A, Anti-B, Anti-D and Anti-AB into corresponding labelled tubes.
3. Add one drop of cell suspension to each tube and mix well.
4. Centrifuge for 1-2 minutes at 1000 RPM or incubate at Room temperature for 20-30 minutes.
5. Gently dislodge cell bottom and observe for agglutination.

INTERPRETATION OF RESULTS:
Agglutination indicates the presence of A and/or B Antigen. No Agglutination is a negative test result and indicates the absence of A and/or B antigen.

Agglutination indicates the presence of Anti-D (Rho)Antigen. No Agglutination is a negative test result and indicates the absence of A nti-D (Rho) antigen.

AGGLUTINATION WITH MONOCLONAL SERA

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