The Alkaline Protease is a solid enzyme produced by submerged fermentation from Bacillus licheniformis according to the specification of GB/T23527-2009 (China). It is widely applied in detergent, leather depilation and softening, silk degumming, Heparin Sodium, Chondroitin and etc.
Definition of Activity:
One gram of solid Alkaline Protease powder at 40°C and pH 10.5 hydrolyze casein in one minute to produce 1μg tyrosine is defined as one enzyme activity unit and marked with u/g.
1. Alkaline Protease can efficiently hydrolyze the protein into free amino acids.
2. Effective temperature: 20-65°C, Optimum Temperature: 40-50°C
Effective pH: 8-12, Optimum pH: 9-11
|Odor||Slight Fermentation Odor|
|Enzyme Activity||100,000 u/g , 200,000 u/g|
|Water Content||≤ 8%|
|Particle Size||≥90% pass 40 mesh sieve|
V. Application & Reference Dosage:
1. Application in Heparin Sodium:
(1).Add salt and alkali at earlier stage. The process is the same with salt dissolving.
(2).Rise the low temperature to 65°C and keep 1 hour.
(3).Cool down the temperature to 45°C and add 3g enzyme powder (100,000 u/g) for one piece of small intestine.
(4).Rise the temperature to 60°C after adding the enzyme and keep 4-6 hours.
(5).Filtering after rising the temperature to 80°C.
(6).Add Resin when the temperature is cooled down to 60°C after the filtering.
(7).The procedure of dissolving salt is different.
Adjust the dosage and the enzymolysis condition according to the quality of raw materials and the practical production conditions.
2. Application in Chondroitin
(1).Stir the enzyme well with water, and then add it into the materials.
(2).Add 0.4%-0.6% of enzyme powder during the whole process of enzymolysis dissolution. Add the enzyme separately by twice. Add 60-70% of the total enzyme in the front two thirds time and add the rest enzyme during the left time.
(3).Enzymolysis condition: Adjust the temperature to 45-60°C and pH at 10.5. Process time: 5-8 hours. Stir once every 30mins.
Adjust the dosage and the enzymolysis condition according to the quality of raw materials and practical production condition.
3. Pretreatment in silk degumming:
Pretreatment → Dehydration → acid treatment (add protease 18-24 u/ml and soap 0.75% at 48-50°C and pH 10.5) → Dehydration → skin tearing off → washing →Dehydration → fluffing → drying → fluffing
4. Application in leather depilation:
Raw Hides → washing → degreasing → alkali expansion → feather plucking → splitting → dealkalization → enzyme depilation → washing → pickling →tanning.
5. Application in detergent:
Decompose protein based stains like grass, blood, mucous, milk, meat, faecal material, human sweat etc. into small, readily soluble peptides and amino acids which can be easily removed during washing process and thus improving the cleaning efficiency of detergents.
Add alkaline protease into the toothpaste, toothpowder, Mouth Refreshner contribute to tartar removing.
Should be stored in a cool, dry and well-ventilated place; protect from direct sunlight, heat and dampness; avoid storing with poisonous and harmful substances.
VII. Shelf Life:
Enzyme activity remains ≥ 90% after 12 months at room temperature 25°C.
20kg/bag, 25kg/bag or as clients’ requirements
IX. Technical Support and Service:
Xingtai Sinobest Biotech Co., Ltd. will provide technical support service in the whole process. Our professional technicians will guarantee every client to use this product under proper guide.
Enzyme preparations belong to protein, which may induce sensitization and cause allergic type reactions in sensitized individuals. Prolonged contact may cause minor irritation for skin, eyes or mucous membrane of nose, so any direct contiguity with human body should be avoided. If irritation or allergic response for skin or eyes develops, please consult a doctor.