DMT Chemically Competent Cell

Product Overview

Description

DMT Chemically Competent Cell

 DMT Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10⁸cfu/μg DNA (tested by pUC19 plasmid DNA).

Cat. No.	CD501
Specification	CD511-01: 5×50 μl
	CD501-02:  20×50 μl
Storage:	at -70oC for six months
Genotype	F- φ80 lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK -, mK +) phoA supE44 thi-1 gyrA96 relA1 tonA
Features	• High transformation efficiency: >108 cfu/μg ( pUC19 DNA).
	• Resistance to T1 and T5 phage.
	• In vivo digestion of methylated DNA, suitable for site-directed mutagenesis.
Procedures	1. Thaw a vial of 50 μl  DMT Chemically Competent Cell on ice, add target DNA into the tube and mix gently. Incubate the cells on ice for 30 minutes.
	2.Heat-shock the cells for 45 seconds at 42oC, and then quickly remove the tube from the 42oC water bath and place them on ice for 2 minutes. Do not shake the tube during this procedure.
	3. Add 500 μl of sterile SOC medium or LB medium (no antibiotic) into the tube, mix well and shake at 37oC for 1 hour at 200 rpm to resuscitate cells.
	4. According to the experiment requirement (plasmid, transformation of recombinant ligation product), add different volumes of transformed cells into corresponding antibiotic-containing LB medium. Spread the transformed cells on selective plate. Invert the plate and incubate at 37oC overnight.
Notes	High efficiency transformation can be achieved by transforming cells immediately following thawing.
	Avoid repeated thawing.
	Avoid pipetting cells.
	Gentle handling is required for the entire procedure.