ProteinIso GST Resin

Product Overview

Description

ProteinIso^TM GST Resin

ProteinIso^TM GST Resin is designed for one-step purification of GST tagged proteins. GST fusion proteins expressed in E.coli, insect cells and mammalian cells can be purified with ProteinIso^TM GST Resin. It is only suitable for soluble protein.

 

Cat. No.	DP201
Specification	DP201-01	10 ml
Storage:	at 2~8oC (20% ethanol) for two years
Notes	To avoid blocking column, sample should be centrifuged, and filtrate with 0.45 μm filter before loading.
	To avoid cross-contamination, we suggest not to use the same medium to purify different proteins.
	Equilibration Buffer 140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7.3
	Elution Buffer 50 mM Tris-Cl pH 8.0, 10 mM reduced glutathione (GSH concentration needs adjustment according to the adhesion strength of the target protein. Glutathione is easy to oxide and prepared just before use).

 

Resin Specifications

References	Specifications
Matrix	4% cross-linked agarose
Ligand	Glutathione
Shape	sphere
Pore size	90 μm
Ligand density	8 mg GSH/ml wet gel
Binding capacity	10-12 mg GST fusion proteins/ml wet gel (MW 42 kDa); 3.5 mg GST/ml wet gel (rat liver)
Maximum flow rate(25oC)	450 cm/h
Recommended flow rate	<150 cm/h
Highest resistance of atmospheric pressure	0.3 Mpa
pH stability	3-10
Chemical stability	Stable in commonly used water-soluble buffer, 70% ethanol, 30% isopropanol, 6M guanidine hydrochloride and 1M acetic acid
Physical stability	Variation rate of resin volume (<2%) affected by pH value of solution or ionic strength

 

Procedures

1. Prepare GST purification column

(1) Resuspend the GST resin in its bottle by inverting.

(2) Transfer the resin into a purification column. Allow the resin to settle.

(3) Equilibrate the column with 5~10 bed volume of equilibration buffer.

2. Prepare samples

To avoid blocking column, samples should be centrifuged and filtrated before loading.

3. Load samples and wash

Load samples and wash with 5~10 bed volume of equilibration buffer and collect the flow-through in one tube.

4. Elute

Elute target protein with elution buffer.

5. Resin Regeneration

(1) 2 bed volume of 6 M GuHCl, 0.2 M acetic acid and 5 bed volume of deionized water or PBS buffer.

(2) 3-4 bed volume of 70% ethanol or 30% isopropanol and 3-5 bed volume of deionized water.

(3) 2 bed volume of 10-100 mM NaOH and 10 bed volume of deionized water.

 

 

 

CITATIONS

Wang T Z, et al. 2013. A Medicago truncatula EF-Hand Family Gene, MtCaMP1, Is Involved in Drought and Salt Stress Tolerance. 8(4):e58952. PLoS One. IF=3.73. PMID: 23593126.