TransScript II All in One First Strand cDNA Synthesis SuperMix for qPCR (One Step gDNA Removal)

Product Overview

Description

TransScript II All-in-One First-Strand

cDNA Synthesis SuperMix for qPCR

(One-Step gDNA Removal)

This supermix provides all the necessary components for cDNA synthesis from total RNA or mRNA. It is provided at 5× concentration and used at 1× concentration by adding gDNA remover, RNA and H₂O. Simultaneous genomic DNA removal and cDNA synthesis are performed in one tube. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85^oC for 5 seconds. . The resulting cDNA are suitable for qPCR and not for regular PCR.

 

Cat. No.	AH341
Specification:	AH341-01	50 rxns (20 μl per reaction)
Storage:	at -20°C for one year
Advantages	Simultaneous genomic DNA removal and cDNA synthesis in one tube.
	The optimal ratio of Oligo(dT)20 Primer to random primer(N9) for qPCR ready cDNA.
	qPCR ready cDNA in 15 minutes.
	cDNA up to 250 bp.
Application	Multiple copy and low copy gene detection
	GC-rich or complex secondary structure RNA template

 

Kit Contents  

 

Components	AH341-01 (50 rxns )
5×TransScript®  II All-in-One SuperMix for qPCR	200 μl
5×TransScript® II All-in-One No-RT Control SuperMix for qPCR	20 μl
gDNA Remover	50 μl
RNase-free Water	1 ml

 

Procedures

Genomic DNA removal and first-strand cDNA synthesis

 1. Reaction Components

Component	Volume
Total RNA/mRNA	*
5×TransScript® II All-in-One SuperMix for qPCR	4 μl
gDNA Remover	1 μl
RNase-free Water	to 20 μl

 

 

qPCR

Reaction Components

Component	Volume	Final Concentration
cDNA	Variable	as required
Forward Primer (10 μM)	0.4 μl	0.2 μM
Reverse Primer (10 μM)	0.4 μl	0.2 μM
2×TransStart® Top Green qPCR SuperMix	10 μl	1×
Passive Reference Dye	0.4 μl	1×
ddH2O	Variable	-
Total Volume	20 μl	-

 

 

 Packaging