High Efficiency TIANamp FFPE DNA Extraction Kit By Xylene Treatment
The TIANamp FFPE DNA Kit applies xylene deparaffinization method to remove paraffin, and provides special lysis condition to extract DNA from formalin-fixed and paraffin-embedding (FFPE) samples. This method overcomes the inhibitory effect of formalin cross-linking. By combining the specific adsorption columns and a special buffer system, high-quality DNA can be extracted into a small elution volume. The purified DNA has good genome integrality, high purity and stable and reliable quality, which is ideally suitable for diagnosis and academic research.
Features:
1.Wide application: The kit could be used to extract gDNA from various FFPE samples.
2.Easy extraction procedure: Easy extraction and purification of high-quality, high-yield and ready-to-use DNA with good repeatability.
3.Stable and reliable: Complete removal of contaminants and PCR inhibitors.
Contents
50 preps
Buffer GA
15 ml
Buffer GB
15 ml
Buffer GD
13 ml
Buffer PW
15 ml
Buffer TE
15 ml
Proteinase K
1 ml
RNase-Free Spin Column CR2 set
50
Collection tubes (2 ml)
50
The genomic DNA extracted by TIANamp FFPE DNA Kit and relevant product from Supplier A were amplified to 190 bp, 330 bp, 500 bp, 650 bp and 1300 bp fragments. The result shows that DNA extracted by TIANamp FFPE DNA Kit has higher yield, higher purity especially for large fragments.
Procedure: Follow the instructions of each product.
Gel loading: gDNA was eluted by 50 μl elution buffer and was amplified to 190 bp, 330 bp 500 bp, 650 bp and 1300 bp. 5 μl of 20 μl PCR products were loaded per lane on 1% agarose gel. The electrophoresis was conducted at 6 V/cm for 20 min.
M: TIANGEN Marker D2000;
T: TIANamp FFPE DNA Kit;
A: Relevant product from Supplier A.
TIANGEN, a leading biotechnology company in China, provides customized molecular biology reagents and professional technical services to customers in the life science research field. Our comprehensive product portfolio includes technologies for Nucleic acid purification products, PCR series reagents ( including regular PCR, RT-PCR and Real time PCR), NGS library preps and NGS service, as well as lab essential instrumentations.
TIANGEN maintains a strict“Customer First”mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.
