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High Effective RNAprep Pure FFPE Kit For Purifying Total RNA
The RNAprep Pure FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions can remove formaldehyde modifications of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase I and Buffer RDD for optimized removal of genomic DNA contamination. The purified RNA can be used in various of downstream applications such as RT-PCR.
Features:
1.Silica membrane based technology to ensure high-purity of RNA.
2.Fast and simple process compared with conventional methods.
3.Suitable for downstream RT-PCR or RT-qPCR applications.
Contents |
50 preps |
Buffer RF |
12 ml |
Buffer RB |
12 ml |
Buffer RW1 |
40 ml |
Buffer RW |
12 ml |
Proteinase K |
500 μl |
RNase-Free ddH2O (Bottled) |
40 ml |
RNase-Free Spin Columns CR3 set (with 2 ml collection tubes) |
50 |
RNase-Free DNase I (1500 U) |
1 |
Buffer RDD |
4 ml |
RNase-Free ddH2O (Tubular) |
1 ml |
RNase-Free Centrifuge Tubes (1.5 ml) |
50 |
Storage:
DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃.
Other reagents could be store at room temperature (15-25℃)
RNA extracted from paraffin-embedded rat liver sample using the RNAprep Pure FFPE Kit.
Sample amount: 15 mg rat liver; Elution volume: 50 μl; Loading volume: 8 μl
M: TIANGEN Marker III
1-4: Rat liver FFPE
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