Best selling cells/ bacteria total RNA isolation extraction kit nucleic acid purification reagent High purity, High yield

Product Overview

Description

Best Selling  RNAprep Pure Bacteria Kit For RNA Extraction

 

   The RNAprep Pure Cell/Bacteria Kit provides a fast, simple and cost-effective method for purification of total RNA from cultured cells and bacteria samples by using effective spin column and unique buffer system. The kit includes RNase-Free Spin Column CR3 for purifying highquality RNA by using silica-membrane technology. High-quality total RNA could be obtained in 30-40 minutes with high-purity and is free of protein and genomic DNA contamination.

Features: 

1.Optimized buffers and protocols for cultured cells and bacteria samples make the process simple and convenient.

2.Unique DNase I minimizes genomic DNA contamination.

3.Unique RNase-Free Filtration Columns CS eliminates other contaminations.

4.The high-purity and ready-to-use RNA is suitable for sensitive downstream applications.

5.No phenol/chloroform extraction, no LiCl and ethanol precipitation, no CsCl gradient centrifugation are needed, which makes the process safe and reliable.

Applications: 

1.RT-PCR.

2.Northern Blot, Dot Blot.

3.Real-Time PCR.

4.Chip analysis.

5.PolyA Screening, in vitro translation, RNase protection analysis and molecular cloning.

Contents	50 preps
Buffer RL	30 ml
Buffer RW1	40 ml
Buffer RW	12 ml
RNase-Free ddH2O (Bottled)	15 ml
RNase-Free Spin Columns CR3 set (with 2 ml collection tubes)	50
RNase-Free Filtration Columns CS set (with 2 ml collection tubes)	50
RNase-Free DNase I (1500 U)	1
Buffer RDD	4 ml
RNase-Free ddH2O (Tubular)	1 ml
RNase-Free Centrifuge Tubes (1.5 ml)	50

Storage:

DNase I, Buffer RDD, RNase-Free ddH2O (Tubular) should be stored at 2-8℃

Other reagents could be store at room temperature (15-25℃) 

Material: Human Jurkat Cells (1×106 )

Method: The total RNA of Human Jukat Cells was isolated using the RNAprep Pure Cell/Bacteria Kit.

Results: Please see the above agarose gel electrophoresis picture. 2-4 μl of 50 μl eluates were loaded per lane. The electrophoresis was conducted at 6 V/cm for 30 min on a 1% agarose.