Best Seller RelaxGene Blood DNA System (0.1-20 ml) For Genomic DNA Extraction
The RelaxGene Blood DNA System adopts a unique buffer system to extract genomic DNA from 0.1-20 ml of fresh blood with various anticoagulants and cryopreserved blood samples. The buffer system can remove the proteins, pigments, lipids and other inhibitory impurities to a maximum extent, and obtain large size DNA fragments with high yield, high purity and good stability. The kit does not need to use organic solvents such as phenol/chloroform. The purified DNA can be used in various routine experiments such as restriction digestion, PCR, library construction, Southern blot (high-throughput sequencing chip hybridization), etc.
Features:
1.Flexible sample starting amount: 2-400 μg high purity DNA can be extracted from 0.1-20 ml of various blood samples.
2. Safe and reliable: No contamination of organic solvents such as phenol/chloroform.
Contents
For 200 ml blood
Buffer CLA
2×250 ml
Buffer FGA
160 ml
Buffer TB
60 ml
Proteinase K
1 ml
Genomic DNA were extracted from blood samples using RelaxGene Blood DNA System.
Samples: 300 μl human anticoagulation blood with EDTA, EDTA/NaF, heparin and sodium citrate; buffy coat from 2 ml blood; 10 μl chicken blood and 200 μl rat blood. 3 μl of 200 μl eluates were loaded per lane. M: TIANGEN Marker D15000.
Experimental results: RelaxGene Blood DNA System can be widely applied in the high-quality DNA purification of human anticoagulants with different treatments as well as the blood from various species.
Purification of the genomic DNA from 300 μl, 5 ml and 10 ml human anticoagulation blood with EDTA
using RelaxGene Blood DNA System. 3 μl of the 200 μl, 500μl and 1 ml eluates respectively were loaded per lane. M: TIANGEN Marker D15000.
Experimental results: 0.1-20 ml of blood can be extracted by RelaxGene Blood DNA System with good linear range.
Comparison of the genomic DNA extracted from 300 μl human anticoagulation with EDTA using RelaxGene Blood DNA System and similar products from other suppliers. M: TIANGEN Marker D15000. 3 μl of 200 μl eluates were loaded per lane.
Experimental results: Compare to other suppliers, RelaxGene Blood DNA System can obtain higher yield, purity and integrality of blood gDNA.
Other Reference Data:
Sample
Storage time
Sample Amount
DNA Yield
OD260 / OD280
Human whole blood
One week at 4℃
300μl
3-10 μg
1.7 - 1.9
Human whole blood
One week at 4℃
1 ml
4-30 μg
1.7 - 1.9
Human whole blood
One week at 4℃
5 ml
100-200 μg
1.7 - 1.9
Human whole blood
One week at 4℃
10 ml
200-400 μg
1.7 - 1.9
TIANGEN, a leading biotechnology company in China, provides customized molecular biology reagents and professional technical services to customers in the life science research field. Our comprehensive product portfolio includes technologies for Nucleic acid purification products, PCR series reagents ( including regular PCR, RT-PCR and Real time PCR), NGS library preps and NGS service, as well as lab essential instrumentations.
TIANGEN maintains a strict“Customer First”mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.
