The basic enzyme-linked immunosorbent assay (ELISA), or enzyme immunoassay (EIA), is distinguished from other antibody-based assays because separation of specific and non-specific interactions occurs via serial binding to a solid surface, usually a polystyrene multiwell plate, and because quantitative results can be achieved.
The ELISA procedure results in a colored end product which correlates to the amount of analyte present in the original sample.
ELISAs are quick and simple to carry out, and since they are designed to rapidly handle a large number of samples in parallel, they are a very popular choice for the evaluation of various research and diagnostic targets. Figure 1 shows a typical ELISA result.
Composition of kit
Microplate coated with Toxoplasma antigen
96 holes
Enzyme labeled anti human IgG antibody
6.5ml
Positive control serum
0.8ml
Critical positive control serum
0.8ml
Negative control serum
0.8ml
Developer A
6.5ml
Developer B
6.5ml
Stop fluid
6.5ml
Concentrated detergent (10 times)
50ml
Sample diluent
52mlx2
Usage and results
Specimen collection
The test was performed with serum or plasma at a dosage of 10 μ L. Do not use bacteria, blood fat, hemolysis or jaundice samples. Collect the serum according to the standard method, and keep the sample at room temperature for no more than 8 hours. If the test is conducted after 8 hours, keep the sample 2 ~ 10 ℃, if stored for more than 1 week, it will be - 20 ℃.
Test procedure
1. Balance the kit at room temperature for 20-30 minutes. The concentrated washing solution shall be diluted by 10 times with distilled water or deionized water.
2. Sample 1:100 dilution: add 1ml of sample diluent into a clean small tube, add 10ml of sample to be tested, and mix well.
3. Fix the battens on the plate frame, seal the remaining battens with adhesive and put them into the sealing bag for storage. Add 100ml diluted sample into each hole, and make at least 2 holes for each sample. Two holes were added for positive control and two holes for negative control, three holes for critical control serum and 100ml holes for critical control serum. Another hole without any liquid was left as blank control. Set at 37 ℃ for 30 minutes.
4. Shake off the liquid in the hole, wash the plate for three times, stay for one minute each time, shake off and pat dry. In addition to the blank control, add 50ml (1 drop) of enzyme marker into each hole, and place it at 37 ℃ for 30 minutes.
5. Shake off the liquid in the hole, wash the plate for 3 times as above, add 50ml (1 drop) of color developing solution a and B to each hole after pat dry, put them at 37 ℃ for 10 minutes, immediately add 50ml (1 drop) of termination solution to each hole, mix them well, and then use the enzyme reader 450nm to read and determine the result.
Result determination
The average value of the critical control a value of sample a value 2 is positive, and the average value of sample a value < the critical control a value is negative.
Our company has strong R & D team, rich experience and strong technical strength. The establishment of a joint laboratory with a number of colleges and universities outside the province, give full play to production, science research, combined the advantages of research focused on quick detection products.
1.Q:Are you a factory or trading company?
A:We are a trading company with our own factory.
2.Q:Where is your factory located? How can I visit there?A:Our factory is located in Nantong City, Jiangsu Province, China, near to the Shanghai. You can fly to Shanghai airport directly .All our clients, from home or abroad, are warmly welcome to visit us!
3.Q:What is MOQ?A: The MOQ depends on diferent products, generally speaking is 20.000pcs but we can do your requirement.
4.Q:How can I get some samples?A: We are honored to offer you some free samples.
5.Q:How does your factory do regarding quality control? A:"Quality is priority. We always attach great importance to quality controlling from the
very beginning to the very end. Our factory has gained FSC, IS013485 and most products have the CE and FDA certificates.
