Hot selling high quality CAS No. 39450 01 6 Proteinase K

Product Overview

Description

China Manufacturer supply Proteinase K CAS No. 39450-01-6 with Lowest price 

Product introduction

What is Proteinase K ?

Proteinase K is a powerful proteolytic enzyme isolated from Candida albicans. It has high specific activity and is a key reagent for DNA extraction. Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

 

Product features 

Proteinase K is a stable and highly reactive serine protease. It cleaves the carboxy terminal peptide bonds of aliphatic amino acids and aromatic amino acids. This enzyme has been purified to remove the activities of RNase and DNase. Because proteinase K is stable in urea and SDS and has the ability to degrade natural proteins, therefore it has a wide range of applications, commonly used in molecular biology, cell biology, immunohistochemistry and other related experiments to digest various proteins, including preparation of chromosomal DNA for pulse electrophoresis, western blotting, and removal of nucleases in DNA and RNA preparation.

 

Functions 

1.Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
2.Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A.
3.Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria.
4.Determination of enzyme localization on membranes.
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
5.Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

 

Product photo

Specs 

Product Name	Proteinase K
Appearance	Liquid
Purity	95%
CAS No.	39450-01-6
Key words	Peptidase K, Endoproteinase K, Endopeptidase K
Application	molecular biology, cell biology, immunohistochemistry
Molecular weight	30kDa
Cart No.	G1205-10ML
Storage	When stored at –20 °C, the product retains activity for at least 1 years, ice bag transportation.
Package	100mg,1ml,10ml and demand by you

 

Package & delivery:

100mg/bottle, 1ml/bottle, 10ml/bottle, when stored at –20 °C, the product retains activity for at least 1 years, ice bag transportation.

 

 

 

Instructions

Can be used in conjunction with our company's TUNEL kit:
Dilute 10 times with PBS (pH 7.4) before usage to prepare proteinase K working solution. Take a certain amount of working solution to cover the tissue,

 

and incubate at 37°C for 10~30 min. Then wash the slides three times with PBS (pH 7.4) in shaker, 5 min each time.

 

Precautions

1. It is recommended to use in separate package, avoid repeated freezing and thawing.
2. Due to the high activity and stability of the proteinase K, cross-contamination in the experiment should be avoided.
3. For your safety and health, please wear lab coats and disposable gloves.

 

Company Profile

Wuhan Servicebio Technology Co., Ltd. Was established in 2007, the headquarter is located in Wuhan, a high-tech enterprise focus on providing technical services and research products in life science field. Servicebio owns more than 1000 employees and more than 40 service centers in large and medium-sized cities across China, it can provide service for people who explore the mysteries of life in life science field.
Servicebio has strong scientific research and service strength, including Antibody R&D Center, Ultra-micro Pathology center, Pathological Testing Center, SPF Laboratory Animal Center and other technology platforms. We do independent research and development to optimize a variety of experimental methods and processes. Meanwhile, developing a variety of reagents and consumables.
we have a professional R&D team to produce and validate antibodies. Laboratory consumables such as centrifuge tubes and tips can be produced on a large scale. Laboratory reagents realize the automated packaging. In this case, we can provide the full range of integrated scientific and technical service in experimental design, method optimization, experimental operation, analytical testing, reagent supplies, etc. For customers.
In quality management system, we have obtained ISO9001-2015 certificate. The instruments manufactured by us have passed CE & FCC certification.