This kit is impregnated with dried monoclonal antibody Anti-1.1 and Anti-1.2 for determining blood type. The antibody in this kit was obtained by injecting specific hybridoma into Balb/C mice. Anti 1.1 and Anti 1.2 showed the molecular weights of approximately 200 KDa and 80 KDa, respectively, as measured by western blot analysis (Lee et al., plos one, 2017).
* Clinical importance of blood types
When canine is transfused with compatible blood type at the first transfusion, the half-life of the blood within the body is long as 21 days. But, when canine is transfused with imcompatible blood type, the half-life of the blood within body is short, approximately 12 hours. (Bull RW: Antigens, grafts rejections, and transfusions. J An Vet Med Assoc 181:1115, 1982) Thus, the blood typing is crucial for successful transfusion for the first time. There are 13 blood types for dogs. Especially, type 1.1 (DEA1.1), and type 1.2(DEA1.2) are very important for transfusion. * 1: If a negative dog is exposed to 1.1 positive erythrocytes, a strong hemolysin can result acute hemolytic reactions. On second exposure, immune-mediated hemolytic transfusion reaction results causing removal of transfused cell in less than 12 hours. * 2: A previously sensitized negative type dog undergoes permanent red blood cell removed and loss 12 to 24 hours after administration of 1.2 positive erythrocytes.
※ Therefore, transfusion must be carried out according to blood type.
* Test procedure 1. Collect 500㎕ of canine blood. (Use EDTA tube, or a tube with anti-coagulant) 2. Put one drop of blood using approximately 20-30㎕ into each well of blood card. 3. Put one drop of PBS(20㎕) each well.
4. Mix with stick (15 seconds) (Use different stick in each well)
5. Examine it within
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