Быстрое тестирование на болезни крупного рогатого скота, комплект для тестирования на антитело Brucellosis (1600176802218)

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Product Overview

Description


Brucellosis  Antibody Elisa test kit



Product Description

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Description


Brucellosis Antibody Test Kit is used to detect the Brucellosis Antibody in serum of bovine, sheep, pig and dog. 


This kit uses competitive ELISA method, Brucellosis antigen is pre-coated on enzyme micro-well strips. When testing, add the serum sample, diluents, Enzyme conjugate. After incubation, if there is Brucellosis specific antibody, it will combine with the pre-coated antigen. Discard the uncombined enzyme conjugate after washing. Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample. When the reaction is terminated by adding stop solution, use ELISA reader at 450nm wavelength to measure the absorbance OD value in reaction wells.


Reagents










































Brucellosis-Ag Coated plate



96 Tests/192 Tests



Stopping solution



15 mL



Enzyme conjugate



22 mL



Negative control



1 mL



Sample diluents



12 mL



Positive control



1 mL



Substrate A (liquid)



12 mL



Adhesive plate sealer



1 pc /2 pcs



Substrate B (liquid)



12 mL



Manual



1pc



wash concentrate(20×)



50 mL



 




Test Procedure:


I. Preparation of reagents


Washing buffer preparation: return 20× concentrated washing buffer into room temperature (20-25)before use. If there is salt crystals, shake to make it dissolved (It is best to incubate 5-10 minutes in laboratory thermostat Incubator). Then dilute it at 20 times with distilled water (for example: 570ml distilled water is added into 30ml wash concentrate) and shake it evenly. The diluted washing buffer can store at 2~8 for about 1 week.


II. Sample preparation


Take animal whole blood, when the blood clot, centrifuge 10 minutes at the speed of 4000rpm. Then take supernatant. The supernatant can also be separated after natural coagulation, but no hemolysis.


III. Operation steps


1.  For every test, set 2 wells for positive control and 2 wells for negative control;


2.  Add serum, negative control and positive control to its corresponding wells, 20μl/well. Do not mix use the tips.


3.  Add sample diluent into each well, 40μl/well.


4. Adding Enzyme Conjugate, 100ul/well, shake gentle to mix it evenly, cover it with Adhesive plate sealer.  Incubate 30 min at 25 in laboratory thermostat Incubator.


5. Open the adhesive plate sealer, discard the liquid of the well, add diluted washing buffer to each/well, 260ul/well. Discard the liquid after 30s. Repeat the above step for 4 times, at last flap to dry with the absorbent paper.


6. Mix substrate A and substrate B evenly by 1:1, add it into each well, 100ul/well. Then cover it with Adhesive plate sealer, incubate 15 min at 25 in laboratory thermostat Incubator.


7. Add stop solution, 50ul/well to stop the reaction, measure the result in 5 min.


 


Result Determination:


Read the OD value at 450nm (630nm as reference) on microplate reader.


Validity criteria


OD value of positive control<0.2, negative control>1.0. Otherwise, the experiment is invalid.


Calculations


PI (blocking rate) =100%- (OD value of samples/ Negative control OD average value) ×100%


Results interpretation


PI>45% is positive


PI ≤45% is negative.


 


 


our advantages:


1. Founded in 2008


2. 200-300 people, 40% technicians


3. 45,000 square meters


4. Quick response rate


5. Same price but better quality and service





Company Information


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Packaging & Shipping


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FAQ


 


Q1: Are you a manufacturer?
Yes, advanced automate machines and professional R&D team ensure the high quality, speedy delivery and large production capacity.
Q2: Can we put our logo/text on the products or package?
Yes, we can do OEM or even ODM. All of our products can be customized.
Q3: Can you provide free samples?
Yes, free samples are available.
Q4: How long is your production time?
Our production time is usually two weeks after receiving the deposit and four weeks for one container order quantity.
Q5: What is the MOQ?
Small quantity like 1 box is available.
Q6: How to make the payment?
By T/T, Western union and Alibaba trade assurance order is also acceptable.




1600176802218
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