Самый популярный набор для тестирования ПЦР в режиме реального времени при Африканской свиней (ASF) для ASFV (1600241826036)

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Product Overview

Description








Real-time PCR for Rapid Detection Kit of ASFV


Product Description

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Description


African swine fever virus (ASFV) real-time PCR test kit enables the safe detection
of African swine fever virus DNA in serum, blood, tissue, lymph nodes, spleen,
muscle from pigs and breeding environment samples within 40 minutes. The kit
consists of fluorescence PCR mix , positive control , negative control and lysis
solution for real-time PCR amplification of ASFV. Fluorescence PCR mix contains
primers, probes, enzyme and buffer for detection of the ASFV P72 gene. In addition,
the role of lysis solution is to simply process samples and release DNA.




 


Operation:


1. Sample preparation
(1) Blood
Option 1: DNA extraction
Follow the commercial DNA extraction kit manual for the DNA extraction.
Option 2: Simple Processing
If the whole blood, serum and plasma samples are fresh and clear, DNA extraction is
not necessary and follow the steps below for the sample preparation:
a. Take 190 μL lysis solution to 2 mL centrifuge tube;
b. Add 10 μL sample to the centrifuge tube, then fully mix;
c. Vortex and take the supernatant.


 


(2) Immune organs
Take 0.1 g sample(about the size of a soybean) to 2 mL centrifuge tube, then add 1
mL sterile saline, grinding and mixing. Centrifugation for 2 minutes with 8000×g,
take out the supernatant.
Option 1: DNA extraction
Follow the commercial DNA extraction kit manual for the DNA extraction.
Option 2: Simple Processing
a. Take the required quantity of centrifuge tubes, add 190 μL lysis solution;
b. Add 10 μL collected samples separately into the centrifuge tube, then mix thoroughly;
c. Centrifuge briefly, take the supernatant.


(3) Muscle tissue
a. Take 0.05 g sample(about the size of a mung bean) to 2 mL centrifuge tube, then
add 1 mL sterile saline, grinding and mixing. Centrifugation for 2 minutes with
8000×g, take out the supernatant.
b. Follow the commercial DNA extraction kit manual for the DNA extraction
(4) Environment sample and others
Follow the commercial DNA extraction kit manual for the DNA extraction.


2. PCR
(1) Take out the reagents in the room temperature for melting, mixing and vortex. Centrifuge briefly.


(2) Dispense 20 μL of fluorescence PCR mix to the appropriate number of PCR strip
tubes.
(3) Add 5 μL of sample supernatant to each well (include the positive control and
negative control for each test). The final reaction volume should be 25 μL.
(4) Seal each PCR tube, then centrifuge briefly to bring the contents to the bottom of
the plate tubes.
(5) Write down the order of each sample.
(6) Set up PCR amplification cycling program as following:


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Result Determination:


(1) Validity Criteria


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(2) Interpretation of Results


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our advantages:


1. Founded in 2008


2. 200-300 people, 40% technicians


3. 45,000 square meters


4. Quick response rate


5. Same price but better quality and service





Company Information


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Packaging & Shipping


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FAQ


 


Q1: Are you a manufacturer?
Yes, advanced automate machines and professional R&D team ensure the high quality, speedy delivery and large production capacity.
Q2: Can we put our logo/text on the products or package?
Yes, we can do OEM or even ODM. All of our products can be customized.
Q3: Can you provide free samples?
Yes, free samples are available.
Q4: How long is your production time?
Our production time is usually two weeks after receiving the deposit and four weeks for one container order quantity.
Q5: What is the MOQ?
Small quantity like 1 box is available.
Q6: How to make the payment?
By T/T, Western union and Alibaba trade assurance order is also acceptable.


 


 




1600241826036
0.0099 s.