PRRS Ab Elisa Test Kit Porcine Reproductive and Respiratory Syndrome Rapid Test Kit (1600312712793)

Share on:


Price:$70.00 - $150.00
Price in USD:Details

Quantity:


Product Overview

Description








Porcine Reproductive and Respiratory Syndrome 

Rapid Test Kit-PRRS Elisa Ab Test Kit


 


 



Product Description

20  20.JPG


Description


Used to detect porcine reproductive and respiratory syndrome virus antibodies in pig serum.


 


Compenents: (96T/ box)


1. 1 piece of coated board
2. Negative control 2m
3. Positive control 2mL
4. Enzyme conjugate concentrate 0.24mL
5. Enzyme Conjugate Diluent 24mL
6. sample diluent 60mL
7. Substrate A solution 12mL1
8. Substrate B solution 12mL
9. Stop solution 15mL
10.25×Concentrated washing sulution 50mL
11. cover film 1 sheet
12. 1 piece of serum dilution plate
13. manual


 


Prepare


Washing solution: return to room temperature (20-25°C), and dissolve the precipitate (preferably heated in a constant temperature incubator at 37°C for 5-10 minutes), and then diluted with deionized water 25 times (for example: 30mL of concentrated washing solution plus 720mL of deionized water) and mix well, and the diluted washing solution can be stored at 2~8°C for 7 days


sample


1 Take whole blood and coagulate, centrifuge it at 4000 rpm for 10 min, and collect the supernatant.


2. Dilute the serum sample to be tested 1:39 (40X) In the serum dilution plate.


 


Operation 


1.Take the coated plate  and 100 μL diluted serum add to the coated plate. At the same time, add 100 μL negative and positive controls to the reaction wells. Gently shake the sample in the well, cover with film, and react for 30 minutes in the dark at room temperature (25±2°C).


2.Discard the solution in the wells, add 300 μL of the diluted washing solution to every well. after standing for 1 min, discard the washing solution. After washing 4 times, pat dry on absorbent paper (must be fully patted dry).


3. Preparation of enzyme conjugate working solution: mix according to the volume ratio of 1:99 (1 part of enzyme conjugate concentrate + 99 parts of enzyme conjugate diluent) for later use. Note: The preparation volume should be determined according to the actual number of samples.


4 Add 100μL of enzyme conjugate working solution to each well, cover with film, and react for 30min in the dark at room temperature (25±2°C).


5 Discard the solution in the well, wash 4 times, the method is the same as.2.


6 Mix the substrate A solution and the substrate B solution 1:1, add to the well, 100μL/well, cover the cover membrane, at room temperature ( 25±2°C) Protect from light and react for 10min.


7 Add 50μL of stop solution to each well, and determine the result within 5 minutes.


8.Use a microplate reader to read the OD value of each well at 450nm (630nm as the reference wavelength).


 


 Read.


POSITIVE: S/P ≥0.10


Suspicious:  0.10 >S/p ≥0.08,  If the sample is still within this range, it will be negative;


Negative: S/p <0.08


 


Other Disease ELISA test kits


 


African swine fever virus antibody detection kit (indirect method)


Porcine foot-and-mouth disease virus non-structural protein Swine fever virus antibody detection kit (indirect method)


Classical swine fever virus antibody detection kit type


Swine fever virus antibody detection kit (indirect method)


Brucella indirect ELISA antibody detection kit (serum)


Brucella indirect ELISA antibody detection kit (milk sample)


Brucella competing ELISA antibody detection kit


Brucella Antibody Detection Kit


 





Company Information


 -1_02.jpg-1_03.jpg





Packaging & Shipping


 -1_04.jpg





FAQ


 


Q1: Are you a manufacturer?
Yes, advanced automate machines and professional R&D team ensure the high quality, speedy delivery and large production capacity.
Q2: Can we put our logo/text on the products or package?
Yes, we can do OEM or even ODM. All of our products can be customized.
Q3: Can you provide free samples?
Yes, free samples are available.
Q4: How long is your production time?
Our production time is usually two weeks after receiving the deposit and four weeks for one container order quantity.
Q5: What is the MOQ?
Small quantity like 1 box is available.
Q6: How to make the payment?
By T/T, Western union and Alibaba trade assurance order is also acceptable.





CONTACT


Sales Manager




Grace Yang Professional in food and safety test, animal disease test field for 3 years

looking for your cooperation.



 


Grace


Wechat/Mobile/WhatsApp: 86-18331598295


Email: wdwkbio(@)gmail.com




1600312712793
0.0082 s.