RNase Free DNase I Set, 9003 98 9, DNA digestion, RNA isolation and purification extraction enzyme
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- Supplier: Guangzhou Magen Biotechnology Co. Ltd.
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Product Overview
Description
Overview
Product Name: RNase Free DNase I Set
Product Use Case: RNA isolation and purification
Packaging Size: 500 Preps/box; 5000 Preps/box
Storage：-20℃
Shelf Life: 2 years
Product Use Case: RNA isolation and purification
Packaging Size: 500 Preps/box; 5000 Preps/box
Storage：-20℃
Shelf Life: 2 years
Order Information
Catalog # | Product Name | Packaging Size | List Price (FOB) |
C12133 | RNase Free DNase I Set | 500 Preps/box | 156 USD |
C12134 | RNase Free DNase I Set | 5000 Preps/box | 1301 USD |
Note: when the quantities of 500 Preps/box are more than 10, we prefer to ship you 5000 Preps/box instead.
Introduction
Bovine pancreatic deoxyribonuclease (also known as DNase) is an endonuclease. It acts on the phospholipid bond, especially the bond adjacent to pyrimidine nucleoside, so as to produce polynucleotides with free hydroxyl at the 3'end and phosphate at the 5' end. The optimum pH value of enzyme action was 7.8. DNase can be activated by divalent metals and inhibited by chelates such as EDTA and sodium dodecyl sulfate. Calcium ion with a concentration of 5 mm can be used as a stabilizer to protect DNase from being decomposed by hydrolase. This product is extracted from bovine pancreas and prepared by chromatography to remove the pollution of other hydrolases.
RNase Free DNase I Set is specially designed for column/magnetic bead RNA extraction kit. Biological samples were lysed, ethanol was added to adjust the binding conditions, and transferred to column or magnetic beads to adsorb RNA. After washing, add DNase I and DNase Buffer to the membrane of the column or magnetic bead, digest at room temperature (25-37℃) for 15 minutes to completely remove the DNA adsorbed on the membrane/beads. After washing away DNase and degraded DNA, elute RNA with DEPC water finally.
RNase Free DNase I Set is specially designed for column/magnetic bead RNA extraction kit. Biological samples were lysed, ethanol was added to adjust the binding conditions, and transferred to column or magnetic beads to adsorb RNA. After washing, add DNase I and DNase Buffer to the membrane of the column or magnetic bead, digest at room temperature (25-37℃) for 15 minutes to completely remove the DNA adsorbed on the membrane/beads. After washing away DNase and degraded DNA, elute RNA with DEPC water finally.
Procedure
In nucleic acid extraction kit based in column, adding this product can carry out DNase I digestion on the membrane to remove DNA contamination
In nucleic acid extraction kit based on magnetic bead, adding this product can perform liquid-phase DNase I digestion to remove DNA contamination
In the crude RNA product, add this product for liquid-phase DNase I digestion to remove DNA contamination
In nucleic acid extraction kit based on magnetic bead, adding this product can perform liquid-phase DNase I digestion to remove DNA contamination
In the crude RNA product, add this product for liquid-phase DNase I digestion to remove DNA contamination
Specifications
Product Name | RNase Free DNase I Set |
Enzyme Activity | 10kunitz Units/ul |
Molecular weight | 32kDa |
Isoelectric point | 8.9 |
pH value range | 4.0-10.0 |
Preservation | -20°C for long term storage |
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