Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR
Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR
Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR
Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR
Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR
Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR

Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA Ultra high fidelity PCR

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Product Overview

Description



Ultra high fidelity PCR mix Fast amplification HIFi polymerase for up to 20 kb DNA








Ultra HiFidelity PCR Kit is a new high fidelity PCR amplification premix suitable for PCR-related cloning and detection.
Ultra HiFi DNA Polymerase contained in the kit is a new fast and high fidelity DNA polymerase developed by directed molecular evolution technology. It enhances the affinity of DNA polymerase to templates, improves the amplification speed and extension ability of the enzyme, and increases the success rate of PCR and product yield.
This product is supplied with the one-tube premix which contains hot-start Ultra HiFi DNA Polymerase, Super pure dNTPs, MgCl2, reaction buffer and other components required for PCR reaction. PCR amplification reaction can be set up by simply adding templates and primers in the premix. The operation is simple and convenient. In addition, PCR Enhancer is supplied in this kit, which can improve the resistance of Ultra HiFidelity PCR Kit to PCR reaction inhibitors and the adaptability to templates with different GC contents. It is suggested to add the PCR Enhancer in the PCR systems for templates with complex structures and with high content of PCR inhibitors.


Features:
1.Easy to operate: This kit is supplied as 2× premix, and PCR can be performed by simply adding templates and primers.
2.High fidelity: The fidelity is 50 times that of Taq polymerase.
3.High specificity: Excellent hot-start performance to ensure product specificity.
4.Rapid amplification: The extension speed can reach 10-15 sec/kb.
5.Strong extensibility: Up to 20 kb DNA fragments can be amplified.
6.Wide applicability: The kit contains PCR Enhancer and is suitable for the amplification of high GC and complex templates.




Contents
4992971
2×UltraHiFi Mix (with dye)
5×1 ml
PCR Enhancer
500 μl
ddH2O
5 ml


Storage:
Store at -20°C.






Figure 1. Ultra HiFi has excellent hot-start function to ensure the specificity of amplification products. Molecular beacons method was applied (Ma et al., Anal Biochem, 2006).




Figure 2. The fidelity of Ultra HiFi is 50 times higher than that of common Taq polymerase. The polymerization fidelity of Taq polymerase (without corrective activity) is used as reference.




Figure 3. Ultra HiFi can extend up to 5 sec/kb for fragments smaller than 4 kb. For long fragments, the amplification time can be extended appropriately. For fragments more than 15 kb, the extent speed can be up to 30 sec/kb. M: TIANGEN D15000 Marker






Strong universality and high specificity, easy to read high GC and long fragments from different sources
Figure 4.Ultra HiFi has high specificity to ensure amplification success rate and productquantity for different types of templates.
A. Ultra HiFi amplification results
B. Hi-Fi enzyme amplification results of Supplier K
C. Hi-Fi enzyme amplification results of Supplier N
M: TIANGEN D15000 Marker
Lane 1-5. Amplification results of templates with different length: 1. 750 bp; 2. 1 kb; 3. 2 kb; 4. 4 kb; 5. 6 kb
Lane 6. Amplification result of high GC template: 1915 bp (GC%: 70%);
Lane 7-11. Amplification result of 2 kb templates from various genome: 7. Rat; 8.
Rice; 9. Wheat; 10. Corn; 11. Bacteria;
Lane 12-14. 8 kb long fragment amplification result: 12. Rice; 13. Corn; 14. Bacteria;
Lane 15. Amplification result of 15 kb long fragment: λDNA (15 kb)




Figure 5. Using Ultra HiFi to amplify fragments with different lengths and concentrations. As low as 1 ng gDNA templates and 1 pg plasmid templates can be well amplified.
M: TIANGEN 1 kb DNA ladder. Lane 1-7. Human gDNA templates: 1. 1000 ng; 2. 500 ng; 3. 100 ng; 4. 50 ng; 5. 10 ng, 6. 5 ng, 7. 1 ng.
M:TIANGEN Marker III. Lane 1-6. 4.5 kb plasmid DNA templates: 1. 100 ng; 2. 10 ng; 3. 1 ng; 4. 100 pg; 5. 10 pg, 6. 1 pg.






TIANGEN, a leading biotechnology company in China, provides customized molecular biology reagents and professional technical services to customers in the life science research field. Our comprehensive product portfolio includes technologies for Nucleic acid purification products, PCR series reagents ( including regular PCR, RT-PCR and Real time PCR), NGS library preps and NGS service, as well as lab essential instrumentations.
TIANGEN maintains a strict“Customer First”mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.












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