Recombinant Ribonuclease A Solution, RNase A, Animal free
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- Supplier: Shenzhen Tinzyme Gene Tech Co. Limited
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Product Overview
Description
Product Description
Ribonuclease A (RNase A) is a genetically engineered endonuclease which is originally from bovine genome. The product is produced and purified from yeast cells without contamination of endotoxin.
RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.
A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.
RNase A is a very stable endoribonuclease and a single chain polypeptide containing four disulfide bridges. Solutions of RNase A have been reported to withstand temperatures up to 100°C. The highest activity is exhibited with single stranded RNA. Activators of RNase A include potassium and sodium salts.
A major application for RNase A is the removal of RNA from preparations of biological products, such as plasmid DNA or protein samples. It is also used in RNA sequence analysis and protection assays.
- CAS No. 9001-99-4
- E.C. 3.1.27.5
- Source Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
- Concentration 10 mg/mL
- Activity ≥ 350 U/μL
- Molecular weight 14.3 kDa
- Form Liquid clear colorless buffered aqueous glycerol solution
- Grade Molecular biology level
- Purity ≥ 90%; (SDS-PAGE)
- Endotoxin None detected (Tachypleus Amebocyte Lysate)
- Foreign activity Endonuclease and exonuclease, none detected
- NICKase and DNase, none detected
- Optimal pH 7.6 (activity range of 6-10)
- Optimal temperature 60°C (activity range of 15-70°C)
- Shelf life 1 year (-20°C)
- E.C. 3.1.27.5
- Source Pichia pastoris cells with cloned gene encoding genetically engineered Bovine pancreatic ribonuclease.
- Concentration 10 mg/mL
- Activity ≥ 350 U/μL
- Molecular weight 14.3 kDa
- Form Liquid clear colorless buffered aqueous glycerol solution
- Grade Molecular biology level
- Purity ≥ 90%; (SDS-PAGE)
- Endotoxin None detected (Tachypleus Amebocyte Lysate)
- Foreign activity Endonuclease and exonuclease, none detected
- NICKase and DNase, none detected
- Optimal pH 7.6 (activity range of 6-10)
- Optimal temperature 60°C (activity range of 15-70°C)
- Shelf life 1 year (-20°C)
Specification
item | value |
CAS No. | 9001-99-4 |
Other Names | RNase A |
EINECS No. | 3.1.27.5 |
Place of Origin | China |
Guangdong | |
Purity | 90% |
Classification | Specific Reagents |
Brand Name | Tinzyme |
Model Number | RA02 |
Concentration | 10 mg/mL |
Activity | ≥ 350 U/μL |
Molecular weight | 14.3 kDa |
Appearance | Liquid: clear colorless buffered aqueous glycerol solution |
Optimal pH | 7.6 (activity range of 6-10) |
Optimal temperature | 60°C (activity range of 15-70°C) |
Storage | -20°C |
Shelf Life | 1 year |
Transportation and Storage
Storage temperature: -20°C.
Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
Dilution buffer: 10 mM Tris-HCl (pH 8.0).
Storage buffer: 10 mM Tris-HCl (pH 8.0), 50% glycerol.
Dilution buffer: 10 mM Tris-HCl (pH 8.0).
Procedure
RNase A is provided as a solution of 10 mg/mL.
RNase A can be used at a final concentration of 10-20 μg/mL.
RNase A can be used at a final concentration of 10-20 μg/mL.
Packing & Delivery
To better ensure the safety of your goods, professional, environmentally friendly, convenient and efficient packaging services will be provided.
Company Profile
Tinzyme is a professional molecular bio reagent Manufacturer in China. The key products are nucleic acid substances and the enzymes for molecular biology.
We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology.
These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology
Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.
We have many patents for the nucleic acid and the enzymes. And the monomers of the nucleic are all enzymatically synthesized using our proprietary unique technology.
These dNTPs and NTPs are functionally approved for all PCR and related reactions including Real Time PCR, in vitro transcription assays and DNA sequencing in diagnostics and molecular biology
Also the enzymes such as Taq DNA polymerase, M-MLV Reverse Transcriptase, pfu DNA polymerase are all worked very well. The performance of our products is comparable to the top bio companies of the world.
FAQ
1. who are we?
We are based in Guangdong, China, start from 2015,sell to Western Europe(13.00%),Eastern Europe(12.00%),North America(12.00%),Southern Europe(11.00%),Northern Europe(11.00%),Central America(11.00%),South America(8.00%),Mid East(5.00%),Oceania(5.00%),Eastern Asia(5.00%),South Asia(5.00%),Southeast Asia(2.00%). There are total about 11-50 people in our office.
2. how can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;
3.what can you buy from us?
Enzymes,dNTP and rNTP,DNA ladder,DNA/RNA purification kit,Proteinase K
4. why should you buy from us not from other suppliers?
We manufacture enzymes and Recombinant enzyme for more than 10 years. We manufacture Proteinase K, DNase I, RNase A, DNA Polymerase and so on.
We also manufacture dNTPs by enzymes, the dNTPs is the top quality comparable to the top 10 bio companies.
5. what services can we provide?
Accepted Delivery Terms: FOB,EXW；
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Escrow;
Language Spoken:English,Chinese
We are based in Guangdong, China, start from 2015,sell to Western Europe(13.00%),Eastern Europe(12.00%),North America(12.00%),Southern Europe(11.00%),Northern Europe(11.00%),Central America(11.00%),South America(8.00%),Mid East(5.00%),Oceania(5.00%),Eastern Asia(5.00%),South Asia(5.00%),Southeast Asia(2.00%). There are total about 11-50 people in our office.
2. how can we guarantee quality?
Always a pre-production sample before mass production;
Always final Inspection before shipment;
3.what can you buy from us?
Enzymes,dNTP and rNTP,DNA ladder,DNA/RNA purification kit,Proteinase K
4. why should you buy from us not from other suppliers?
We manufacture enzymes and Recombinant enzyme for more than 10 years. We manufacture Proteinase K, DNase I, RNase A, DNA Polymerase and so on.
We also manufacture dNTPs by enzymes, the dNTPs is the top quality comparable to the top 10 bio companies.
5. what services can we provide?
Accepted Delivery Terms: FOB,EXW；
Accepted Payment Currency:USD,EUR,CNY;
Accepted Payment Type: T/T,MoneyGram,PayPal,Western Union,Escrow;
Language Spoken:English,Chinese
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