2. Clean and disinfect the vulva of the mother with warm water. After washing, wipe the vulva with a wet cloth to ensure
that there is no residue left
3. Put the thawed fine tube into the sperm insemination gun in the traditional way for standby;
4.Turn on the switch of the visual insemination gun. After confirming that the image is clear, insert the gun into the vagina of the mother. Initially, insert the gun into the vagina at an angle of 5-10 cm, then change it to a horizontal position, and continue to insert about 25 cm until the cervix can be clearly seen;
5. Keep the visual insemination gun still, insert the vas deferens with frozen fine tubes into the insemination channel of the visual insemination gun, observe on the screen to ensure that the head of the vas deferens has reached the uterine orifice
6.After reaching the uterine orifice, gently rotate the vas deferens into the uterus of the mother. When the vas deferens is inserted into the uterine mouth about 10 cm, it reaches the bottom of the uterine body. Then the semen in the vas deferens is slowly pushed to the maternal uterus until the push is completed. (Note: if the vas deferens is inserted too deep, semen may only be released to one side of the uterine horn, resulting in uneven distribution of semen. If the other side of the uterine horn rehearsal, it will directly affect the conception rate);
7. After confirming that the insemination is correct, slowly pull out the insemination gun, click the switch to make the visual insemination gun vibrate, promote the contraction of the cow uterus, and facilitate the pregnancy of the cow. Then, pull the visual insemination gun away from the mother's vagina, wipe it with a clean wet cloth and disinfect it with alcohol cotton. Finally, pack the gun and put it into the designated container for proper storage.