Foregene factory supply ivd raw material ForeDirect RT QPCR Kit lab reagent for RNA direct real time amplification

Product Overview

Description

 

Description:

 

ForeDirect RT-qPCR Kit is designed for RNA direct real-time amplification from swab collections without RNA purification processes. This kit completes the qRT-PCR cycles within one hour. The enzyme mix is an optimized blend of Reverse Transcriptase, Hot-Start Taq DNA polymerase, RNase inhibitor. The Reaction Buffer contains all of the required components, including optimized buffer components, Mg²⁺, dUTP, and dNTP_s.

Specifications:

 

100Rxns (20μl system)

 

 

Kit components:

 

ForeDirect RT-qPCR Kit
Catalogue number/specification	DRT-02011/100T
Nucleic acid release agent	500 μL
RNA protectant	50 μL
2× RT-qPCR Buffer	1500 μL
Enzyme Mixture(Taq&M-MLV)	150 μL
Instruction	1 ea

 Note: Components from different kits cannot be used interchangeably

 

PCR Application:

1. Once the PCR reaction tubes have been briefly centrifuged, place them in the sample tank of amplification instrument.

2. Thermal protocol

Note: Fluorescent signal was detected immediately after the extension step of each cycle.

3. After setting, save the file and run the reaction program.

 

Sample requirement

 

1. Applicable samples: Nasopharyngeal Swab or Oropharyngeal Swab

2. Sample collection: 

Use only synthetic fiber swabs with plastic shafts. Do not use calcium alginate swabs or swabs with wooden shafts. Place swabs immediately into sterile tubes containing 1~3 mL of viral transport media. Viral transport media containing guanidine can not be used.

Nasopharyngeal Swab: Insert a swab into nostril parallel to the palate. Swab should reach depth equal to distance from nostrils to outer opening of the ear. Leave swab in place for several seconds to absorb secretions. Slowly remove swab while rotating it.

Oropharyngeal Swab: Swab the posterior pharynx, avoiding the tongue.

3. Storage:The collect specimens should be tested as soon as possible. If storage is required, specimens can be store at 2- 8℃ for no more than 24 hours;

Transport: The specimen should be transported using sealed foam box with ice pack

 

Storage and Shelf life:

 

- The kit should be stored at -20 ± 5℃. The validity period is 12 months.

- Avoid repeated freeze-thaw cycles (<5 cycles).

 

  Foregene Co., Ltd is specialtized in the IVD kits and R&D reagents production and services, which is integrated in R&D, production and marketing. Foregene has the world’s leading Direct PCR platform.The main products include various IVD kits, nucleic acid purification reagents,PCR and Direct PCR reagents,Genotyping kits, etc. The technology and product performance indicators used are all leading the world status,widely applied in hospitals, institutions, medical laboratories, etc.

 

  Foregene Co., Ltd. was founded in April 2011 by well-known scholars who returned from studying at Yale University, famous inventors and senior industry professionals.Foregene’s R&D team consists of 40 people(mainly phD and master), has 2500 m² comprehensive building, 600 m² P2 laboratory, and 1200m² GMP production facility.

With Direct PCR technology, there’s no need for cumbersome nucleic acid purification, the sample is directly added to the system and then on the machine.Now all indicators of Foregene’s Direct PCR technology meet the needs of clinical diagnosis.