Food feed grain Quantitative Aflatoxin B1 rapid detection reagent/aflatoxin test kit
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- Supplier: Quicking Biotech Co. Ltd.
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Product Overview
Description
Product Description
Aflatoxin B1 B2 G1 G2 Rapid Test Kit Strip for milk feeds corn wheat bean flour | ||
*Brand | Shanghai Quicking Biotech | |
*Kit Component | - 10×foil pouches, each containing one AFB1 cassette with a pipette and a desiccant - 1×assay diluent (50 mL) - 2×centrifugal tubes (15 mL) - Products Manual | |
*Result | negative, positive ,invalid | |
*Accu | 98% | |
INTENDED USE
Quicking Afaltoxin B1 Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Aflatoxin B1
residue in feed, grain or spices samples.
Cut-off: 5 ppb base
Assay Time: 5 - 10 min
Sample: Feed, grain or spices extraction
residue in feed, grain or spices samples.
Cut-off: 5 ppb base
Assay Time: 5 - 10 min
Sample: Feed, grain or spices extraction
LIMITATION
Quicking Aflatoxin B1 Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its
convenience. It provides a semi-quantitative method to detect the Aflatoxin B1 above 5 ppb in feed, grain or spices sample. If you
want a quantitative result, please adopt other method such as ELISA/ HPLC in practice.
Quicking Aflatoxin B1 Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its
convenience. It provides a semi-quantitative method to detect the Aflatoxin B1 above 5 ppb in feed, grain or spices sample. If you
want a quantitative result, please adopt other method such as ELISA/ HPLC in practice.
Information Need to Know
Aflatoxin B1
Aflatoxin B1 is an aflatoxin produced by Aspergillus flavus and A. parasiticus. It is a very potent carcinogen with a TD50 3.2 μg/kg/day in rats.This carcinogenic potency varies across species with some, such as rats and monkeys, seemingly much more susceptible than others. Aflatoxin B1 is a common contaminant in a variety of foods including peanuts, cottonseed meal, corn, and other grains;as well as animal feeds. Aflatoxin B1 is considered the most toxic aflatoxin and it is highly implicated in hepatocellular carcinoma (HCC) in humans.[citation needed] In animals, aflatoxin B1 has also been shown to be mutagenic,[9] teratogenic, and to cause immunosuppression.Therefore, there is great demand for aflatoxins research to develop suitable methods for their quantification, precise detection
and control to ensure the safety of consumers’ health
and control to ensure the safety of consumers’ health
Test Procedure &Result Read
TEST PROCEDURE
* Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 0 g
ground portion of the sample into the centrifugal tube (15 mL).
* Add 2 mL of pure water and 8 mL of ethyl acetate into the tube. Shake for 10 min. (If emulsification appears, do a
centrifugation at 4000 rpm for 1 min.)
* Collect accurately 4 mL of supernatant (ethyl acetate layer) into a small container (beaker or tube). Dry the liquid by
blowing wind.
* Redissolve the residues in the small beaker with an amount of the provided assay diluent. (REF: Table )
* Take out the cassette from the foil pouch and place it horizontally.
Suck the liquid and drip 3 drops into the assay sample hole “S”. If the migration has not appeared after 30 seconds, add one more
ground portion of the sample into the centrifugal tube (15 mL).
* Add 2 mL of pure water and 8 mL of ethyl acetate into the tube. Shake for 10 min. (If emulsification appears, do a
centrifugation at 4000 rpm for 1 min.)
* Collect accurately 4 mL of supernatant (ethyl acetate layer) into a small container (beaker or tube). Dry the liquid by
blowing wind.
* Redissolve the residues in the small beaker with an amount of the provided assay diluent. (REF: Table )
* Take out the cassette from the foil pouch and place it horizontally.
Suck the liquid and drip 3 drops into the assay sample hole “S”. If the migration has not appeared after 30 seconds, add one more
Cut-off (ppb) | 5 | 10 | 20 | 50 | 100 |
Assay Diluents (mL) | 0.4 | 0.8 | 1.6 | 4.0 | 8.0 |
* Take out the cassette from the foil pouch and place it horizontally.
Suck the liquid and drip 3 drops into the assay sample hole “S”. If the migration has not appeared after 30 seconds, add one more
Suck the liquid and drip 3 drops into the assay sample hole “S”. If the migration has not appeared after 30 seconds, add one more
* drop of the liquid into sample hole.
* Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
* Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
Company Introduction
shanghai Quicking Biotech Co., Ltd. (Quicking Biotech), established in 2006, is a biological high-tech leader in innovative
products and services for product rapid detection. Our company, located in Pudong New Area, Shanghai, China, focus on researching and developing the most advanced food safety rapid diagnostic products and veterinary rapid test kits for companion animal and farm animals
products and services for product rapid detection. Our company, located in Pudong New Area, Shanghai, China, focus on researching and developing the most advanced food safety rapid diagnostic products and veterinary rapid test kits for companion animal and farm animals
Flow chart & QC
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