Bacteria Total RNA Purification Kit Through Spin Column Method

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Bacteria Total RNA Purification Kit (Spin Column)


[Product Code ]


208141


[Specification]


50 reactions


[Introduction]


The kit can be used to extract total RNA from cultured Gram-negative and Gram-positive bacteria. With the purification technology of silica membrane, the kit can complete extraction in 30-40 mins, avoid the application of phenol, chloroform and toxic agents, and eliminate DNA, impure protein and other impurities to the maximum extent. The kit is applicable to multiple downstream experiments such as RT-qPCR, chip analysis, Northern Blot, Dot Blot, PolyA filtration, in vitro translation, RNase protection analysis and molecular clone.


[Contents]























































Product CompositionContentQuantity
Buffer TE15 mL / bottle1 bottle
Proteinase K1.04 mL / tube1 tube
Buffer BARB20 mL / bottle1 bottle
Buffer BARW36 mL / bottle1 bottle
Buffer CFW212 mL / bottle1 bottle
RNase-Free Water7 mL / bottle1 bottle
Spin Column NP2050 pieces / bag1 bag
Collection tube50 pieces / bag1 bag
Instructions1 copy1 copy

[Shipping & Storage Conditions and Shelf Life]


Transported at room temperature and stored at room temperature away from light. The shelf life is 12 months.


Production Date: Refer to the label.


Expiration Date: Refer to the label.


[Equipment and Reagents Prepared by User]


1. Lysozyme


2. DNase I (optional)


3.  Absolute ethyl alcohol


4. Centrifugal tube of 1.5 mL


5.  Vortex mixer


6.  Centrifugal machine: maximum speed of revolution 12,000 rpm (~13,400×g)


[Reagent Preparation]


1. Dissolve lysozyme by Buffer TE until its concentration reaches 15 mg/ mL and store the dissolved lysozyme at temperature of -20 °C. Please prepare lysozyme yourself for it is not available in the kit. (Lysozyme of Sangon Biotech, Order No.: A610308; Sigma, Cat. No. L7651 is recommended).


Attention: Lysozyme should not be frozen or thawed repeatedly. It is suggested to pack the dissolved lysozyme separately before storage or use it promptly upon preparation. Other enzymes may be more effective and applicable to some bacteria. (Lysostaphin, for example, is more effective and applicable to rupture cytoderm of staphylococcus aureus.)


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About us




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About us



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ABOUT US


Wuxi NEST Biotechnology Co., Ltd.





Company Profile


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A leading life science plastic consumables manufacturer.Wuxi NEST Biotechnology Co., Ltd. a leading life science plastic consumables manufacturer, who is integrated with R&D production and sales, was established in 2009, located in Wuxi, Jiangsu, China. Our products have been exported to North America, Europe, Japan, Korea, India and other countries, enjoys an excellent reputation nationwide and abroad. Customers are almost all over the world.2011, NEST passed the standard of quality management system of ISO 9001.2014, NEST passed the standard of quality management system of ISO 11137.2016, NEST passed the standard of quality management system of ISO 13485.2020, NEST obtained the medical device production license.In addition to these certifications, we also gained CE standard.NEST Scientific Inc. NJ, USAWith the development of the business, in order to send the products to customers in North America more quickly, establish deeper cooperation and build strong relationships with oversea customers. NEST Scientific Inc. as a branch of Wuxi NEST Biotechnology Co., Ltd. established in New Jersey, USA in 2013. Composed by a professional team with rich experience in training and sales skills, can provide professional training, communicate with customers in depth and understand customers’ demands more quickly.



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In addition to these certifications, we also gained CE standard.



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NEST Scientific Inc. NJ, USA With the development of the business, in order to send the products to customers in North America more quickly, establish deeper cooperation and build strong relationships with oversea customers. NEST Scientific Inc. as a branch of Wuxi NEST Biotechnology Co., Ltd. established in New Jersey, USA in 2013. Composed by a professional team with rich experience in training and sales skills, can provide professional training, communicate with customers in depth and understand customers’ demands more quickly.






OUR FACTORY


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Advantage




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Advantage



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Excellent mold-making ability.


Our R&D team, with over 30 years’ experiences, work with high quality mold-making equipment imported from Germany and professionalmold analysis software that allow NEST to develop more precise molds.




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Injection molding in Class 10,000 and Class 100,000 clean rooms, with strict quality control.


10,764 ft2 Class 10,000 clean room and 32292 ft2 Class 100,000 clean room, all-electric high-speed injection molding machines imported from Japan, raw material which meet USP Class VI requirements. Production and quality control are performed strictly in accordance with corresponding SOP.




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Sterilization by electronic beam irradiation.


The Rhodotron TT200 electron accelerator which imported from the IBA company, Belgium. A world leader in the E-Beam sterilization industry. Sterilization process has been certified by the ISO 11137 quality system. Compared with sterilization by cobalt 60 or ethylene oxide sterilization, sterilization by electronic beam irradiation is more efficient and safer. It requires less time and will not produce any chemical residue.









Shipping & payment




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Shipping & payment



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We have a professional research and development team to design, produce and export. Also, we have a perfect after-sales service system to work for our clients.


 








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FAQ




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FAQ




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Q1: Are you a factory or trading company?


A1: We are a manufacturer based in Wuxi, Jiangsu, China.




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Q2: What is your hot items?


A2: We are professional in the field of laboratory plasticware especially in cell culture related products.




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Q3: How about the Shipping Method?


A3: DHL/UPS/TNT/Fedex and other air shipments and sea shipments are all workable. In one words, we could do any shipments 




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Q4: How about the delivery date?


A4: Please consult our sales people about the lead time.




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Q5: How about the MOQ ?


A5: Please consult our sales people about the MOQ.









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