GDSBIO Highly efficient and stable One step Probe RT qPCR Kit V3 V5011
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- Supplier: Guangzhou Dongsheng Biotech Co. Ltd.
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Product Overview
Description
One-step Probe RT-qPCR Kit V3
Cat. No.:V5011
Components
Component | V5001 (200 rxn, 30 μl/rxn) |
5 × One-step U+ Mixa | 4ml × 2 |
One-step U+ Enzyme Mix V3b | 1ml × 2 |
a. Contains dNTP/dUTP Mix, Mg2+.
b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.
Storage
This reagent should be kept at -20°C.
Description
The One-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 2 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.
Applications
• Probe gene expression analysis
• Probe Low-copy gene detection
• Probe microarray validation
• Probe gene knockdown validation
Features
• One-step RT-qPCR
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification
• dUTP/UDG system, effectively prevent PCR product contamination
Notes
1. One-Step U+ Enzyme Mix contains high concentration of glycerol. Before use, please centrifuge briefly, collect to the bottom of the reaction tube, and gently suck with a pipetting gun.
2. For preparation of reaction solution, please use RNase-free pipette tips, EP tubes, etc., to avoid contamination as far as possible.
Related products
Founded in 2005, Dongsheng Biotech Co., Ltd is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products. Our company has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents and equipment for nucleic acid extraction and detection, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.
Dongsheng has realized that product quality is the lifeblood of an enterprise from the very beginning. Targeted and standard production process and quality control system have been established for each type of product to ensure product scale production and quality stability.
Dongsheng is the first batch of private biotech enterprises certified by ISO9001 system in China as early as 2010. At the same year, Dongsheng products began to enter overseas markets, and welcomed by overseas customers for excellent uniformity and stability. At present, our products have been exported to more than 20 countries and regions of Asia, Europe and the Americas and received a very good international reputation because of excellent products and professional technical support.
Dongsheng has now grown into one of the highest quality molecular biology reagent manufacturers in China after 12 years of domestic and international market testing, and will continue to contribute to the cause of life science.
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