Blood DNA Midi Kit (1 5ml) Blood Isolation Midi Kits For DNA From Blood

Product Overview

Description

Descripation:

 

The blood sample contains a large number of red blood cells and platelet cells, which makes the blood processing more troublesome. However, the blood genomic DNA extracted by the general method is not high in purity and the content is too low. This kit uses the brand-new Foregene Protease Plus and unique BL1 and BL2 buffer systems, which can completely digest anticoagulated blood and coagulation samples in the shortest time, thereby avoiding DNA degradation to the greatest extent and obtaining the largest amount of genomic DNA. The fast blood processing system and simple spin column operation greatly simplify the extraction of blood genome, so that high-quality, high-purity genomic DNA can be obtained within 40 minutes.

The DNA-only silica gel membrane used in the spin column of this kit is a unique new material of the company, which efficiently and specifically adsorbs DNA, and can remove RNA, impurity proteins, ions and other organic compounds in cells to the greatest extent. The obtained DNA fragments are large, with high purity, stable and reliable quality, and the maximum carrying capacity of a spin column is 80 μg DNA. The obtained DNA can be used for molecular biology experiments such as restriction digestion, PCR, Southern hybridization, library construction and so on. The kit can process up to 5ml of blood at a time to obtain a sufficient amount of high-purity genomic DNA.

 

Kit Contents:

 

Blood DNA Midi Kit
Kit contents	DE-05121	DE-05122	DE-05123
	50 T	100 T	250 T
Buffer BL1	25ml	50ml	125ml
Buffer BL2	25ml	50ml	125ml
Buffer DC	500ml	500ml x2	500ml x5
Buffer PW	25ml	50ml	125ml
Buffer WB1	15ml	30ml	75ml
Buffer EB	10ml	20ml	50ml
Foregene Protease Plus	2.5ml	5ml	12.5ml
DNA-Only Column	50 sets	100 sets	250 sets
IFU	1 piece	1 piece	1 piece

 

Features:

 

- No RNase contamination: The DNA-Only Column provided by the kit makes it possible to remove RNA from genomic DNA without adding RNase during the experiment, avoiding the laboratory from being contaminated by exogenous RNase.

- Fast speed: Foregene Protease Plus has higher activity than similar proteases, and it digests tissue samples quickly; the operation is simple, and the genomic DNA extraction operation can be completed within 40 minutes.

- Convenient: The centrifugation is performed at room temperature, and there is no need for 4°C low-temperature centrifugation or ethanol precipitation of DNA.

- Safety: No organic reagent extraction is required.

- High quality: The extracted genomic DNA has large fragments, no RNA, no RNase, and extremely low ion content, which can meet the requirements of various experiments.

 

Kit Application:

 

It is suitable for extraction and purification of genomic DNA from fresh or frozen anticoagulated whole blood.

 

Genomic DNA Application:

 

The genomic DNA purified by the Blood DNA Midi Kit has high purity and can be used for routine molecular biology operations, such as restriction enzyme digestion, PCR, Southern hybridization, library construction and other experiments.

 

DNA-Only Column Characters:

 

Maximum binding capacity	80μg
Maximum loading volume	800μl
Longset DNA fragment	23kb
Minimum elution volume*	100μl
Selection of samples	Fresh anticoagulated whole blood
Maximum amount of starting material*	5ml

*: The minimum elution system of 100μl is a reasonable recommended volume considering the DNA recovery rate and concentration. If you want to increase the yield of DNA, you can increase the volume of the eluate, if you want to increase the concentration of the purified DNA, you can reduce the volume of the eluate appropriately, such as using a 50μl elution system, while sacrificing part of the DNA yield. In order to get a higher concentration of DNA.

 

Work Flow:

 

 

Storage conditions:

 

This kit can be stored for 12 months under dry conditions at room temperature (15-25℃); if it needs to be stored for a longer period of time, it can be stored at 2-8℃.

Note: If stored at low temperature, the solution is prone to precipitation. Before use, be sure to place the solution in the kit at room temperature for a period of time. If necessary, preheat it in a 37°C water bath for 10 minutes to dissolve the precipitate, and mix it before use.

Foregene Protease Plus solution has a unique formula, which is active when stored at room temperature for a long time (3 months), its activity and stability will be better when stored at 4°C, so it is recommended to store it at 4°C, remember not to keep it at -20℃.