TGuide Smart Magnetic Plant RNA Kit fit with TGuide S16 Nucleic Acid Extractor purify high quality total RNA from Plant Tissues
- Category: >>>
- Supplier: Tiangen Biotech (Beijing) Co. Ltd.
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Product Overview
Description
Product Description
TGuide Smart Magnetic Plant RNA Kit
This product adopts magnetic beads and a unique buffer system to isolate and purify high quality total RNA from plant tissues. The uniquely embedded magnetic beads have a strong affinity for nucleic acid under certain conditions. When the conditions are changed, the magnetic beads can release the absorbed nucleic acid to rapidly separate and purify it.
It can be used to perfectly fit with TGuide S16 Nucleic Acid Extractor. Through absorption, transfer and release of magnetic beads by the special magnetic bar, magnetic beads and nucleic acid can be transferred to improve the degree of automation. The whole process is safe and convenient. The total RNA extracted has good purity and high yield.
Total RNA purified by this kit can be used for RT-PCR, qPCR, chip analysis, Northern Blot, Dot Blot, Poly A screening, in vitro translation, RNase protection analysis, molecular cloning and other downstream experiments.
Product Detail
1. Simple and fast: Ultra-pure total RNA can be obtained by running TGuide S16 for 58 minutes.
2. Flexible throughput: It can perfectly fit with TIANGEN TGuide S16 Nucleic Acid Extractor to extract 1-8 samples.
3. Safe and non-toxic: No toxic reagents such as phenol/chloroform
4. High purity: The RNA obtained has high purity and can be directly used in downstream experiments such as chip detection and high-throughput sequencing.
Automation workflow
The installation and operation require little training. With pre-installed programs, unpack the cartridge, select the protocol and run your experiment. Programs are ready-to-use, and also customizable. Preloaded reagents and matched disposable consumables. The chemical handling is eliminated to the greatest extent to ensure the operation safety.
Experimental Example
Wheat root total RNA extraction
Sample size: 100 mg
Sample pretreatment: grinding with liquid nitrogen or low temperature tissue homogenizer treatment
Agarose gel concentration: 2%(TAE)
Loading volume: 2 μl
Marker: Marker IV, TIANGEN
Experiment result: using TGuide S16 achieved with reagent 4993552 to automatically extract the total RNA of wheat roots has good yield and high purity. In this experiment, about 20 μg of nucleic acid was extracted from 100 mg wheat roots, with OD260/OD280 around 2.0~2.1, and OD260/OD230＞2.0.
Total RNA extraction from different plant samples
Sample size: 100 mg
Sample pretreatment: low temperature homogenizer
Agarose gel concentration: 1% (TAE)
Loading volume: 1 μl
M: Marker III, TIANGEN
1-4: corn seeds; 5-8: corn leaves; 9-12: cabbage roots; 13-16: soybeans
The first two samples were extracted by spin-column extraction kit and the last two samples were extracted by TGuide S16.
Experiment result: the extraction yield of plant RNA by TGuide S16 with reagent 4993552 is equivalent to or better than that by the spin-column extraction kit. The purity and integrity are basically the same. Thus, TGuide S16 can be used to extract plant total RNA in replacement of the spin-column based extraction solution.
Company Profile
TIANGEN, a leading biotechnology company in China, provides customized molecular biology reagents and professional technical services to customers in the life science research field. Our comprehensive product portfolio includes technologies for Nucleic acid purification products, PCR series reagents ( including regular PCR, RT-PCR and Real time PCR), NGS library preps and NGS service, as well as lab essential instrumentations.
TIANGEN maintains a strict “Customer First” mentality and is dedicated to a continuous improvement in quality to fulfill our promise of best-in-class products and services.
FAQ
Q: What are the specific categories of your products?
A: From sample preparation, purification to downstream gene expression, analysis and detection, TIANGEN has corresponding reagents, instruments and even automation platform for customers from both academic institutes and industrial corporations.
Q: What’s your production capacity?
A: We have the production capacity of 1 million tests of kits per month.
Q: Do you have any certificates?
A: YES, we have all the certificates（ISO13485, ISO9001, CE, NMPA ）required for export and local import custom clearance.
Q: Do you support OEM/ODM?
A: YES, we can prototype and customize the ideal product according to your specific application.
Q: What's your lead time?
A: For on-shelf product, the lead-time is 7 days. For customized product, the lead-time will be 14-30 days according to the
ordering amount.
Q: Do you have MOQ?
A: For on-shelf product, we don't have MOQ limit, you can order any quantity you want. For customized products, you can set the scales with your specification, logo, packing, etc. so the MOQ shall be negotiated case by case.
Q: What are your acceptable payment methods?
A: T/T Business to business account
Q: What's your position in the industry?
A: TIANGEN has been established for 16 years, and is the leading upstream supplier company in the molecular biology industry of China.
Q: Which countries and regions have your products been exported to?
A: We have exported our products to 40 countries in Asia, America, Europe and Africa.
Q: Can I have a visit to your factory before the order?
A: Sure, we sincerely welcome you to visit our factory.
Our services
We provide more than OEM service. Our one station customized service help you gain stronger competitiveness in the future market.
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