GDSBIO Highly efficient and stable dNTP/dUTP One step Probe RT qPCR Kit V2 V5009/V5010

Product Overview

Description

One-step Probe RT-qPCR Kit V2

Cat. NO.:V5009,V5010

Components

Component	V5009 (200 rxn, 30 μl/rxn)	V5010 (5000 rxn, 30 μl/rxn)
2X RT-qPCR Mix *	760 μl × 4	38.4 ml × 2
Enzyme Mix b	308 μl	7.7 ml

Description

The One-step Probe RT-qPCR Kit V2 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Thermostable reverse transcriptase can maintain stable activity at 55°C. The hot-start antibody-modified fast DNA polymerase and optimized buffer system ensure the extremely high sensitivity and specificity of the One Step Probe RT-qPCR Kit V2. The kit is available as a convenient Master Mix. 2 × RT-qPCR Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan®.

 

 

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Founded in 2005, Dongsheng Biotech Co., Ltd is a high-tech enterprise focusing on R&D, production and sales of high-quality life science products. Our company has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, nucleic acid electrophoresis, nucleic acid purification, it has developed reagents and equipment for nucleic acid extraction and detection, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.

Dongsheng has realized that product quality is the lifeblood of an enterprise from the very beginning. Targeted and standard production process and quality control system have been established for each type of product to ensure product scale production and quality stability. 

Dongsheng is the first batch of private biotech enterprises certified by ISO9001 system in China as early as 2010. At the same year, Dongsheng products began to enter overseas markets, and welcomed by overseas customers for excellent uniformity and stability. At present, our products have been exported to more than 20 countries and regions of Asia, Europe and the Americas and received a very good international reputation because of excellent products and professional technical support.

Dongsheng has now grown into one of the highest quality molecular biology reagent manufacturers in China after 12 years of domestic and international market testing, and will continue to contribute to the cause of life science.