MH RT PCR kit Mycoplasma hyopneumoniae Real Time PCR Detection Kit
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- Supplier: Beijing Wdwk Biotechnology Co. Ltd.
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Product Overview
Description
MH RT-PCR kit- Mycoplasma hyopneumoniae Real-Time PCR Detection Kit |
Product Description
Description
Mycoplasma hyopneumoniae real-time PCR test kit enables the safe detection of
Mycoplasma hyopneumoniae DNA in respiratory tract secretion swab, lung
tissue from pigs and bacterial cultures within 60 minutes. The kit consists of
fluorescence PCR mix,positive control and negative control for real-time PCR
amplification of Mycoplasma hyopneumoniae. Fluorescence PCR mix contains
primers, probes, enzyme and buffer for detection of the Mycoplasma
hyopneumoniae p36 gene.
Respiratory tract secretion swab,
Lung tissue and
Bacterial culturesserum(all need DNA extraction )
[Components]
Fluorescence PCR Mix | 1.2mL | - 2 0℃ |
| Positive Control | 1 tube | - 2 0℃ |
| Negative Control | 1 tube | - 2 0℃ |
| Instruction manual |
PCR
(1) Take out the reagents in the room temperature for melting, mixing and vortex.
Centrifuge briefly.
(2) Dispense 20 µL of fluorescence PCR mix to the appropriate number of PCR strip
tubes.
(3) Add 5 μL of sample supernatant to each well (include the positive control and
negative control for each test). The final reaction volume should be 25 μL.
(4) Seal each PCR tube, then centrifuge briefly to bring the contents to the bottom of
the plate tubes.
(5) Write down the order of each sample.
(6) Set up PCR amplification cycling program as following:
| Volume | 25 μL | |||
Signal channel | FAM signal channel to record fluorescence | |||
| Step | Temperatures | Time | Cycles | |
Amplification | 1 | 50℃ | 2min | 1 |
| 2 | 95℃ | 3min | 1 | |
| 3 | 96℃ | 10sec | 45 | |
| 56℃ | 20sec | |||
The Interpretation of Results please contact salse.
contact(@)wdwkbio.com
Tel/ Wechat: 8617600617680
WA: 8618331598295
our advantages:
1. Founded in 2008
2. 200-300 people, 40% technicians
3. 45,000 square meters
4. Quick response rate
5. Same price but better quality and service
Company Information
Packaging & Shipping
FAQ
Q1: Are you a manufacturer?
Yes, advanced automate machines and professional R&D team ensure the high quality, speedy delivery and large production capacity.
Q2: Can we put our logo/text on the products or package?
Yes, we can do OEM or even ODM. All of our products can be customized.
Q3: Can you provide free samples?
Yes, free samples are available.
Q4: How long is your production time?
Our production time is usually two weeks after receiving the deposit and four weeks for one container order quantity.
Q5: What is the MOQ?
Small quantity like 1 box is available.
Q6: How to make the payment?
By T/T, Western union and Alibaba trade assurance order is also acceptable.
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