INTENDED USE The Brucella melitensis Antibody Rapid Test is a lateral flow immunochromatographic assay for the qualitative detection of Brucella melitensis Antibody in goat’s serum, plasma, or whole blood.
PRINCIPLE The Brucella melitensis Antibody Rapid Test is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated Brucella LPS antigens. If there are anti-Brucella antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of Brucella melitensis antibodies in the specimen.
TEST PROCEDURE 1. Allow all kit components and sample to reach room temperature prior to testing. 2. Collect the goat’s fresh whole blood, serum or plasma specimen for use. Immediately apply the testing once collected the specimen. 3.Take out the test device from the foil pouch and place it horizontally. 4. Add 20μL of serum or plasma specimen into an assay buffer tube. Mix well and directly use in the assay. 5. Add 2-3drops (approx.60-80μL) of assay buffer into the sample hole. 6. Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
INTERPRETATION OF RESULTS
Positive: Two lines appear, no matter T line is clear or vague. Negative: Only C line appears. Invalid: No line appears or only T line appears.
PRECAUTIONS
1. Expired test or the test with damaged foil pouch should not be used. 2. The test must be used within 10 minutes after opening the foil pouch. 3. Do not use specimen with hemolysis or be contaminated with microbes, which will cause false positive or false negative result. 4. Do not reuse the test kit and do not mix components from different lot numbers. 5. All specimens have potential infection. It must be strictly treated according to the rules by local states.
LIMITATION SheNungtex Brucella melitensis Antibody Rapid Test is for in vitro veterinary diagnosis use only. All results should be considered with other clinical information available from veterinarian. For an accurate result, it is suggested to apply other method such as PCR for final determination in practice.
Why Choose SheNungtex?
ISO9001 & GMP Standard Factory
Shenungtexis a rapid test kit brand of Guangzhou Daoke Biomedical Technology Co., Ltd.
We specialize in developing and manufacturing veterinary diagnostic detection kit for animal health care.
Our factory has professional and experienced facility and technical ability to innovate a series of diagnosis test kit for animal diseases.
SheNungtex aim is to provide high-quality and consistence products for animal diagnostic solution.