Amoxicillin ELISA Test Kit milk safety detection 96wells/kit ISO9001

Product Overview

Description

Amoxicillin ELISA Test Kit

Catalog No. LSY-10054

 

 

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Amoxicillin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Amoxicillin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti- Amoxicillin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Amoxicillin in it. This value is compared to the standard curve and the Amoxicillinconcentration is subsequently obtained.

 

2. Technical specifications

Sensitivity: 0.2 ppb

Incubation Temperature: 25℃

Incubation Time: 30min—15min

Detection limit:

Tissue, Egg, Milk............................................................................... 4ppb

Note: ppb=ng/mL or ng/g

Cross-reaction rate:

Amoxicillin........................................................................................ 100%

Recovery rate:

80%±15%

 

3. Components

1)  Micro-well strips: 12 strips with 8 removable wells each

2)  Standard solution (1ml/bottle): 0ppb, 0.2ppb, 0.6ppb, 1.8ppb, 5.4ppb, 16.2ppb.

3)  11X Concentrated Enzyme conjugate (0.7 mL)

4)  Enzyme conjugate dilution (7 mL)

5)  Substrate A (7 mL)

6)  Substrate B (7 mL)

7)  Stop solution (7 mL)

8)  20× concentrated washing buffer (30 mL)

9)  Sample diluent (20 mL) 

 

4. Materials required but not provided

1)    Equipments: microplate reader (450nm, 630nm), rotary evaporator/nitrogen-drying device,homogenizer, oscillator, centrifuge (3000g and above), balance (a sensibility reciprocal of 0.01 g), measuring pipets, incubator (adjustable 25℃),timer

2)    Micropipettors: single-channel 20~200 µL and 100~1000 µL, and eight-channel 30~300 µL;

3)    Reagents: Deionized water, Concentrated hydrochloric acid (analytical pure)

 

5. Sample pre-treatment

Instructions

The following points must be dealt with before the pre-treatment of any kind of sample:

1) Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents;

2) Before the experiment, each experimental equipment must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results.

Solution preparation before sample pre-treatment:

1)    Washing buffer: 1 part 20× concentrated washing buffer + 19 parts deionized water;

2)    1M HCl: Take 8.6 mL Concentrated hydrochloric acid, add deionized water to 100mL.

5.1 Tissue

1) Take 1.0±0.05g tissue sample into 10ml Polystyrene centrifuge tube; add 4ml deionized water, vortex in high-speed for 1min;centrifuge at above 3000g at room temperature (20 - 25 ℃) for 5min;

2) Take 200ul up-layer clear liquid into 2ml Polystyrene centrifuge tube, add 200ul Sample diluent, vortex for 10s and mix it evenly;

3) Take 50 µL for analysis.

       Fold of dilution of the sample: 10

5.2 Egg

1) Take 1.0±0.05g egg sample into 10ml Polystyrene centrifuge tube; add 4ml deionized water, then add 150uL 1M HCl, vortex in high-speed for 1min;centrifuge at above 3000g at room temperature (20 - 25 ℃) for 5min;

2) Take 200ul up-layer clear liquid into 2ml Polystyrene centrifuge tube, add 200ul Sample diluent, vortex for 10s and mix evenly;

3) Take 50 µL for analysis.

       Fold of dilution of the sample: 10

5.3 Milk

1) Take 1.0mL milk sample into 10ml Polystyrene centrifuge tube; add 4ml deionized water, then add 40uL 1M HCl, vortex in high-speed for 1min;centrifuge at above 3000g at room temperature (20 - 25 ℃) for 5min;

2) Take 200ul up-layer clear liquid into 2ml Polystyrene centrifuge tube, add 200ul Sample diluent, vortex for 10s and mix evenly;

3) Take 50 µL for analysis.

       Fold of dilution of the sample: 10

 

3. Components

1	Micro-well strips	12 strips with 8 removable  wells each
2	6× standard solution (1mL each)	0ppb	1ppb
		3ppb	9ppb
		27ppb	81ppb
3	Enzyme conjugate	7ml	red cap
4	Antibody working solution	7ml	blue cap
5	Substrate A	7ml	white cap
6	Substrate B	7ml	black cap
7	Stop solution	7ml	yellow cap
8	20× concentrated washing buffer	40ml	white cap
9	20×concentrated redissolving solution	50ml	transparent cap

 

 

 

Our Antibiotic Residues ELISA test kits

  

Product Code	Product Name	Spec	Sensitivity (ppb)	Sample Performance
Food Safety ELISA test kit(Antibiotic,veterinary drugs and toxin analysis)
Antibiotic Residues
LSY-10001	Nitrofuran (AMOZ) ELISA Test Kit	96 well/kit	0.03	Fish, Shrimp, Chicken/Liver, Honey, Egg, Feed
LSY-10002	Nitrofuran (AOZ) ELISA Test Kit	96 well/kit	0.01	Fish, Shrimp, Chicken/Liver, Honey, Egg, Feed
LSY-10003	Nitrofuran (AHD) ELISA Test Kit	96 well/kit	0.04	Fish, Shrimp, Chicken/Liver, Honey, Egg, Feed
LSY-10004	Nitrofuran (SEM) ELISA Test Kit	96 well/kit	0.02	Fish, Shrimp, Chicken/Liver, Honey, Egg, Feed
LSY-10005	Neomycin ELISA test kit	96 well/kit	0.1	Muscle tissue, liver,milk, milk powder, feed, duck,honey, egg, pig urine
LSY-10006	Tetracyclines(TCs) ELISA Test Kit	96 well/kit	0.05	Chicken, Pork, Honey
LSY-10007	Chloramphenicol(CAP) ELISA Test Kit	96 well/kit	0.015	Aquatic products, Honey, Meat, liver, Egg, Feed, milk, milk powder
LSY-10008	Florfenicol ELISA Test Kit	96 well/kit	0.1	Aquatic products, Animal tissue, honey,milk
LSY-10069	Thiamphenicol ELISA Test Kit	96 well/kit	0.1	Tissue
LSY-10009	Sulfonamides residues (SAs) ELISA Test Kit	96 well/kit	0.4	Aquatic products, Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine, milk
LSY-10010	Sulfamethazine (SM2) ELISA Test Kit	96 well/kit	1	Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine, milk
LSY-10011	Sulfamethoxydiazine (SMD) ELISA Test Kit	96 well/kit	1	Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine
LSY-10015	Sulfaquinoxaline(SQX) ELISA Test Kit	96 well/kit	1	Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine, milk
LSY-10038	Sulfamethoxazole (SMZ) ELISA Kit	96 well/kit	1	Aquatic products, Chicken/Liver, Pork/Liver, Honey, Egg, Serum, Urine, milk
LSY-10055	Sulfadiazine(SDZ) ELISA Test Kit	96 well/kit	1	Aquatic products, Chicken/Liver, Pork/Liver, Honey, Serum, Urine, milk
LSY-10016	Quinolones(QNS) ELISA Test Kit Fluoroquinolone ELISA Test Kit	96 well/kit	1	Fish, shrimp, Meat, Honey, liver, serum
LSY-10017	Enrofloxacin ELISA Test Kit	96 well/kit	1	Fish, shrimp, Meat, Honey, liver, serum
LSY-10062	Ciprofloxacin ELISA Test Kit	96 well/kit	1	Fish, shrimp, Meat, Honey, liver, serum
LSY-10064	Ofloxacin ELISA Test Kit	96 well/kit	0.1	Tissue
LSY-10018	Ampicillin ELISA Test Kit	96 well/kit	0.1	Fish, shrimp, Meat, Honey, liver, milk
LSY-10019	Benzyl penicillin ELISA Test Kit	96 well/kit	0.05	Fish, shrimp, Meat, Honey, liver, milk
LSY-10067	Cefalexin ELISA Test Kit	96 well/kit	0.3	Tissue, egg, liquid milk, milk powder
LSY-10054	Amoxicillin ELISA Test Kit	96 well/kit	0.2	Tissue, egg
LSY-10020	Tylosin ELISA Test Kit	96 well/kit	0.5	Tissue
LSY-10023	Gentamicin ELISA Test Kit	96 well/kit	0.05	Tissue, Liver, milk
LSY-10024	Streptomycin ELISA Test Kit	96 well/kit	0.2	Tissue/Aquatic product, honey, milk,milk powder
LSY-10027	Malachite green ELISA kit	96 well/kit	0.05	Fish, shrimp
LSY-10044	Oxytetracyline ELISA Test Kit	96 well/kit	0.4	Tissue, honey, millk ,feed
LSY-10049	Chlortetracyline ELISA test kit	96 well/kit	0.2	Tissue, honey, milk
LSY-10051	Doxycycline ELISA test kit	96 well/kit	0.1	Tissue, honey
LSY-10045	Lincomycin ELISA Test Kit	96 well/kit	0.1	Tissue, feed, honey
LSY-10047	Nitroimidazoles ELISA Test Kit	96 well/kit	0.05	Tissue, milk, egg
LSY-10048	Metronidazole elisa test kit	96 well/kit	0.05	Honey
LSY-10061	Dimitridazole ELISA Test Kit	96 well/kit	0.05	Pork, Chicken
LSY-10050	Erythromycin ELISA test kit	96 well/kit	0.2	Milk, honey, tissue
LSY-10053	Tilmicosin ELISA test kit	96 well/kit	0.5	Chicken, Pork
LSY-10056	Colistin ELISA Test Kit	96 well/kit	1.5	Chicken, duck, milk, feed
LSY-10057	Azithromycin ELISA Test Kit	96 well/kit	0.05	Tissue, egg, milk
LSY-10058	Kanamycin ELISA kit	96 well/kit	0.5	Pork, Chicken, honey
LSY-10059	Spectinomycin ELISA kit	96 well/kit	0.3	Tissue, honey
LSY-10065	Trimethoprim ELISA Test Kit	96 well/kit	0.5	Tissue
LSY-10072	Apramycin Residue ELISA test Kit	96 well/kit	0.05	Tissue, honey, egg, milk, milk powder

 ISO9001:2008 certificate

 

 

Company Information

  

We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.

 

Our advantages at the Diagnostic kits/ ELISA test kits field are as following:

 

* ISO9001&ISO13485 certificated company

* Own a high-quality R&D team with many years of experience on bio-diagnostic field.

* Professional manufacturer of ELISA test kits, offer OEM service.

*At present, our products have been exported to over 100 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.

*Delivery time: 1-3 days after confirmation by FedEx, DHL or other cheaper ways.