A filter with a cutoff zone on both sides of the transmission band of the spectral characteristic curve is called a bandpass Filter. Bandpass filter is an important optical thin film element, which has been widely used in chemistry, spectroscopy, laser, astrophysics, optical fiber communication, biology and other fields. The spectral curve of the bandpass filter is prepared by the principle of light wave interference, the wavelength near the spectral transmission region is called the passband of the filter, usually both sides are the cut-off zone, there may be a bypass band around the cut-off area, it is usually necessary to use colored glass, absorbing film or cut-off filter to eliminate the bypass band 405nm laser is replacing expensive ultraviolet lasers for most biological work. The most commonly used lasers are the 488 nm blue-green argon laser, the 543 nm helium-neon green laser, and the 633 nm helium-neon red laser. Mixed gas lasers, such as krypton-argon lasers, can output multiple laser lines, so filters may still be required for selective excitation