Tinzyme BsaI GMP Grade Restriction Endonucleases Derived from Bacillus Thermophilus High Quality Chemical Reagents

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Product Overview

Description



Product Description


Restriction endonucleases are a class of endonuclease that recognize specific deoxynucleotide sequences and cleave the
phosphodiester bond between two deoxyribonucleotides at specific sites in each chain. BsaI derived from Bacillus Thermophilus, is a commonly used restriction endonuclease. BsaI recognizes the following sequence:
5’…GGTCTC(N)1↓…3’;
3’…CCAGAG(N)5↑…5’.
Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid
contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
This product has completed the DMF record of FDA.


Unit Definition


At 37℃, pH 7.5, within 1 h, the amount of enzyme required for totally digestion 1μg λDNA (HindIII digestion, none Dcm
methylation) is defined as one unit of enzyme activity.


Order information


Item
Quantity
BsaI, GMP Grade (10U/μl)
100μl


Component


Components
Quantity
10×BsaI Reaction Buffer, GMP Grade
5μl
Substrate DNA
1μg
BsaI, GMP Grade
1μl
RNase Free Water
Up to 50μl


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