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Green Spring Cefalexin ELISA Test Kit
Catalog No. LSY-10067
1. Principle
This test kit is based on the indirect competitive enzyme immunoassay for the detection of Cefalexin in samples. The coupling antigens are pre-coated on the micro-well stripes. The Cefalexin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Cefalexin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Cefalexin in the sample. This value is compared to the standard curve and the Cefalexin concentration is subsequently obtained.
2. Technical specifications
Sensitivity: 0.3 ppb
Incubation Temperature: 25℃
Incubation Time: 30min—15min
Detection limit:
Tissue 10ppb
Egg 15ppb
Liquid milk 5ppb
Milk powder 24ppb
Cross-reaction rate:
Cefalexin 100%
Recovery rate
Tissue 100%±30%
Egg, liquid milk, milk powder 90%±30%
3. Components
1 | Micro-well strips | 12 strips with 8 removable wells each | |
2 | 6× standard solution (1mL each) | 0ppb | 0.3ppb |
0.9ppb | 2.7ppb | ||
8.1ppb | 24.3ppb | ||
3 | High concentration standard | 1ml | 1 ppm |
4 | Enzyme conjugate | 7ml | red cap |
5 | Antibody working solution | 7ml | blue cap |
6 | Substrate A solution | 7ml | white cap |
7 | Substrate B solution | 7ml | black cap |
8 | Stop solution | 7ml | yellow cap |
9 | 20× extracting buffer A | 15ml | yellow cap |
10 | 2× extracting buffer B | 50ml*2 | transparent cap |
11 | 20× concentrated washing buffer | 15ml | white cap |
12 | 10× concentrated redissolving solution | 10ml | black cap |

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We are Shenzhen Lvshiyuan Biotechnology Co.,Ltd (www.lsybt.com), professional manufacturer and exporterof the most advanced Food Safety ELISA test kit(e.g. Nitrofuran (AMOZ, AOZ, AHD, SEM) ELISA kit, Clenbuterol ,Ractopamine ELISA kit), Animal Disease Diagnostic kits(e.g. Swine FMD lgG Distinguish kit,PRRS,PRV ELISA kit, NDV,IB,IBD ELISA kit),small molecule antigen and antibodies, gene recombination antigen and antibodies, etc.
Our advantages at the Diagnostic kits/ ELISA test kits field are as following:
* ISO9001&ISO13485 certificated company
* Own a high-quality R&D team with many years of experience on bio-diagnostic field.
* Professional manufacturer of ELISA test kits, offer OEM service.
*At present, our products have been exported to over 40 countries, such as United States, Belgium, France, Korea, Germany, Singapore etc.
*Delivery time: 1-3 days after confirmation by FedEx,EMS or other cheaper ways.
ISO9001 Certificate





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