S&CBiotech Feline Infectious Peritonitis Virus Ab Test is alateral flow immunochromatographic assay for the qualitative detection of Feline Infectious Peritonitis Antibody (FIPV Ab) in cat’s serum, pleural fluid and ascitic fluid specimen. Assay Time: 5-10 min Sample: serum, pleural fluid and ascitic fluid
2. PRINCIPLE OF THE ASSAY
S&C Biotech Feline Infectious Peritonitis Virus Antibody Rapid Test is based on sandwich method lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated FIPV recombinant antigens. If there are FIPV antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of FIPV
3. KIT COMPONENT
* 10 test pouches, with cards and disposable droppers * 10×assay buffer tubes * Product Manual
4. TEST PROCEDURE
* Allow all materials, including specimen and test device, recover to 15-25℃ before running the assay. * Take out the test device from the foil pouch and place it horizontally. * Collect 20μL of the prepared specimen into a vial of assay buffer and mix well. Then drop 3 drops (approx.120μL) of the diluted sample into the sample hole “S” of the test card. *Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
5. INTERPRETATION OF RESULTS
Positive: The presence of both C band and T band, no matter T band is clear or vague.
Negative: Only clear C band appears.
Invalid: No colored band appears in C zone, no matter whether T band appears.
6. STORAGE
The kit can be stored at room temperature (4-30°C). The test kit is stable through the expiration date (24 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.
7. SPECIMEN PREPARATION AND STORAGE
- Specimen should be obtained and treated as below. Serum or plasma: collect the whole blood from the patient dog, centrifuge it to get the plasma, or place the whole blood into a tube which contains anticoagulants to get serum. - All specimens should be tested immediately. If not for testing right now, they should be stored at 2-8℃.
8. PRECAUTIONS
* For best results, please strictly adhere to these instructions. * All reagents must be at room temperature before running the assay. * Do not remove test cassette from its pouch until immediately before use. * Do not reuse the test kit. * Do not use the test beyond its expiration date marked on the foil pouch. * The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
Recommend Products
Packing & Delivery
Why Choose Us
About US
1. S&C is a professional bio-tech products manufacturer,and a Specialist in the development, manufacture.
Factory & Technology
2, We pride ourselves in owning state-of-the-art production lines, technical expertise, R & D ability and in the manufacturing process is supported by our highly-skilled R & D team
Lab
3, S&C offers wide range of Rapid immunodiagnostic products for point of care testing, PCR test kits and ELISA products for veterinary diseases.