TransStbl3 Chemically Competent Cell

Product Overview

Description

TransStbl3 Chemically Competent Cell

Trans Stbl3 Chemically Competent Cell is specifically designed for chemical transformation of DNA. It permits a transformation efficiency of over 10⁸cfu/μg DNA (tested by pUC19 plasmid DNA).

 

Cat. No.	CD521
Specification	CD521-01: 10×100 μl
Storage:	at -70oC for six months
Genotype	F- mcrB mrr hsdS20(rB -, mB -) recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20 (StrR) xyl-5 λ- leu mtl-1
Features	• Suitable for lentivirus and retrovirus vector replication.
	•  Minimize the error recombination.
Procedures	1. Thaw a vial of 100 μl Trans Stbl3 Chemically Competent Cell on ice, aliquot 50 μl of the cells into a prechilled 1.5 ml tube, add target DNA into the tube and mix gently. Incubate the cells on ice for 20-30 minutes.
	2. Heat-shock the cells for 45 seconds at 42oC, and then quickly remove the tube from the 42oC water bath and place them on ice for 2 minutes. Do not shake the tube during this procedure.
	2. Add 500 μl of sterile SOC medium or LB medium (no antibiotic) into the tube, mix well and shake at 37oC for 1 hour at 200 rpm to resuscitate cells.
	3. According to the experiment requirement (plasmid, transformation of recombinant ligation product), add different volumes of transformed cells into corresponding antibiotic-containing LB medium. Spread the transformed cells on selective plate. Invert the plate and incubate at 37oC overnight.
Notes	• High efficiency transformation can be achieved by transforming cells immediately following thawing.
	• Avoid repeated thawing.
	• Avoid pipetting cells.
	• Gentle handling is required for the entire procedure.