ПФУ ДНК полимераза, высокоточная ПЦР

Product Overview

Description

Description

Pfu DNA polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has superior thermostability and proofreading properties compared to other thermostable polymerase. Its molecular weight is 90 kDa. It can amplify DNA target up to 2 kb(simple template). The elongation velocity is 0.2~0.4kb/min(70~75°C). Pfu DNA polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA polymerase is superior for techniques that require high-fidelity DNA synthesis.

 

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

 

Storage Buffer

20mM TrisCl ( pH8.0), 100mM KCl, 3mM MgCl₂ 1mM DTT,0.1% NP-40 ,0.1% Tween20, 0.2mg/ml BSA, 50% (v/v) glycerol

 

10X Pfu Buffer with MgSO₄ 

200mM Tris-HCl(pH8.8,25°C), 100mM KCl, 100mM (NH₄)₂SO₄,  20mM MgSO₄, 1.0% Triton^®X-100, 1mg/ml BSA

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Applications

• High-fidelity PCR and primer-extension reactions.

• High fidelity PCR for cloning into blunt-ended vectors.

• Site-directed mutagenesis.

 

Store all components at –20°C

 

 

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Dongsheng Biotech PCR Reagents are looking for distributors and providing OEM service

Dongsheng Biotech offers different series of products to help you achieve PCR success. For enzymes, our Taq Polymerase, HS Taq DNA Polymerase, FS Taq DNA Polymerase, Pfu DNA Polymerase and Fusion Pfu DNA Polymerase provide high fidelity, efficient, sensitivity PCR performance. We also have Long Taq DNA Polymerase for long PCR performance.