Eco Friendly Plasmid Miniprep Kit Spin Column DNA Purification Kit

Product Overview

Description

Eco-Friendly plasmid miniprep kit

For research use only

Content	N1021	N1022	N1023
RNase A(10 mg/ml)		300 ul	600 ul
Solution I	1.5 ml	15 ml	30 ml
Solution II	5 ml	30 ml	60 ml
Solution III	5 ml	30 ml	60 ml
Wash Buffer Q	3 ml	30 ml	30 ml×2
Wash Buffer W	3 ml×2	30 ml×2	40 ml×3
Eluent Buffer	1 ml	10 ml	20 ml
Spin Column	10	100	200

 

Before starting:Add RNase A to solution I according to instructions on the label. Mix well. Store buffer at 4°C. Solution I of N1021 has already been premixed with RNase A, can be used directly.Add 1.5 volume of 96%-100% ethanol to 1 volume of Wash Buffer Q and Wash Buffer W . If the solution II contains salt precipitates, warm the buffer in a 37°C water bath for a feminutes until precipitates dissolve. Do not shake the buffer. If the solution II contains salt precipitates, warm the buffer in a 37°C water bath for a feminutes until precipitates dissolve. Do not shake the buffer.

 

Description:The Eco-Friendly system is the only method available that does not utilize Guanidine Salt, a hazardous protein denaturant, in the silica based purification system, which will ensure the safety and health of scientists and the environment.

 

Features:High purity: No chemicals that may cause protein degeneration; No Guanidine-HCl, CsCl, inon exchange resin, and EB; Enough for multiple applications.
High yield: 1-5ml bacterial suspension yield 10-40 μg plasmid DNA;
Time saving: Purify your DNA in less than 30min
Safety and environmental protection: No toxic chemicals in the solution, which is safer for people and environment.

 

Store :RNase A: store at -20°C. Before starting, please add RNase A to Solution I, mix well and store at 4°C.Other reagent can be store at room temperature.

 

If precipitate forms in the buffers during storage, it should be redissolved by incubating the buffers at 37°C before use.