Marker 11, DNA ladder, OEM M1131/M1132

Product Overview

Description

Item No.	M1131	M1132
Size	50ug	5x50ug

Concentration: 75ng/µl         

 

Recommended Loading

2-5 µl/Lane.

 

Concentration

Typical Bands     125 ng/5µl

Other Bands      50 ng/5µl

 

Recommended Electrophoresis Condition

5 µl/Lane, 8 cm 1.7 %Agarose Gel, 0.5×TBE, 7V/cm, 1h.

 

Contents(bp)

200 ,400,600,800,1,000,1,500

 

Storage

1 year at room temperature, for long-time preservation, please storage at -20°C .

 

Description

Marker 11 is ideal for determining the size of double-stranded DNA from 200 to 1,500 base pairs. The ladder consists of 6 linear double-stranded fragments .The 800bp fragment is present at increased intensity to allow easy identification. All fragments are precisely quantified and mixed during the production. For 5 ul loading, all fragments except 800bp are 50ng. The 800bp fragment is 125ng. This ladder is pre-mixed with loading dye and is ready to use.

 

Recommendations

Do not heat before loading.

For quantification, adjust the concentration of the sample to equalize

it approximately with the amount of DNA in the nearest band of the ladder.

Visualize DNA by staining with ethidium bromide or with SYBR^® Green I.