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2*EasyTaq PCR SuperMix for PAGE
EasyTaq® PCR SuperMix for PAGE is a ready-to-use mixture of EasyTaq® DNA Polymerase for PAGE, dNTPs and optimized buffer. The supermix for PAGE is provided at
2× concentration and used at 1× concentration by adding template, primers and H2O.
| Cat. No. | AS112 | ||
| Storage: | at -20°C for two years | ||
| Ordering Information: | Mix(+dye) | AS112-11 | 1 ml |
| AS112-12 | 5×1 ml | ||
| AS112-13 | 15×1 ml | ||
| Features | The extension rate is about 1-2 kb/min. | ||
| Unique buffer system compatible with PAGE. | |||
| Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be cloned into pEASY ®-T vectors. | |||
| Amplification of genomic DNA fragment up to 3 kb. | |||
| Advantages | High efficiency amplificatio | ||
| Excellent stable performance | |||
| Application | Short fragment amplification | ||
| Notes | Completely thaw the contents in the tube and mix well before each use. | ||
Reaction Components
| Component | Volume | Final Concentration |
| Template | Variable | as required |
| Forward Primer (10 μM) | 1-2 μl | 0.2-0.4 μM |
| Reverse Primer (10 μM) | 1-2 μl | 0.2-0.4 μM |
| 2×EasyTaq® PCR SuperMix for PAGE | 25 μl | 1× |
| ddH2O | Variable | - |
| Total volume | 50 μl | - |



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