Rohi Afaltoxin B1 Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Aflatoxin B1 residue in feed, grain or spices samples.
Cut-off: 5 ppb base
Assay Time: 5 - 10 min
Sample: Feed, grain or spices extraction
2.PRINCIPLE OF THE ASSAY
Rohi Aflatoxin B1 Rapid Test is based on competitive lateral flow immunochromatographic assay. The Aflatoxin B1 conjugate in the test zone will capture the immuno-gold (colloid gold-AFB1 antibody conjugate), when there is very little dissociative AFB1 in the extraction. A visible red test band in T zone indicates a negative result. The test band (T zone) will be not visible if AFB1 is present in above cut-off value concentration in samples, which explains a positive result.
3.KIT COMPONENT
-20×foil pouches, each containing one AFB1 cassette with a pipette and a desiccant
-2×assay diluent (50 mL)
-4×centrifugal tubes (15 mL)
-Products Manual
4.TEST PROCEDURE
-Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 2.0 g ground portion of the sample into the centrifugal tube (15 mL).
-Add 2 mL of water and 8 mL of ethyl acetate into the tube. Shake for 5 min. (If emulsification appears, do a centrifugation at 4000 rpm for 1 min.)
-Collect accurately 2 mL of supernatant (ethyl acetate layer) into a small container (beaker or tube). Dry the liquid by blowing wind.
-Redissolve the residues in the small beaker with an amount of the provided assay diluent. (REF: Table )
Cut-off (ppb)
5
10
20
50
100
Assay Diluents (mL)
0.4
0.8
1.6
4.0
8.0
-Take out the cassette from the foil pouch and place it horizontally.
-Suck the liquid and drip 3 drops into the assay sample hole “S”.
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
5.INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Aflatoxin B1 is at or above 5 ppb in the samples.
Negative: The presence of both bands in C zone and T zone.
Invalid: No band appears in C zone.
6.STORAGE
The test kit is stable for 18 months when stored at room temperature (2-30°C). DO NOT FREEZE and AVOID SUNLIGHT.
7.PRECAUTIONS
-For best results, please strictly adhere to these instructions.
-All reagents must be placed at room temperature before running the assay.
-Do not remove test cassette from its pouch until immediately before use.
-Do not reuse the test kit.
-Do not use the test beyond its expiration date marked on the foil pouch.
-The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.
8.LIMITATION
Rohi Aflatoxin B1 Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Aflatoxin B1 above 5 ppb in feed or grain samples. If you need a quantitative result, please adopt other method such as ELISA/ HPLC in practice.
9.REMARK
Further application notes for nuts, chilli powder, edible oil and fermented wine are available on request.
Packaging & Shipping
8 * 11 * 15 cm / box, 0.16 kg / box,By Sea, SHANG HAI PORT
Our Services
Rohi Biotech dedicate to developing rapid test kit for agricultural field, including agricultural products detection,food safety inspection, animals health, and medical rapid diagnostic use.
Rohi has a modernproduction qualificationof immunochromatography, ELISA,
immunofluorescence R&D center and 400 square meters purification workshop is designed in full accordance with GMP, excellent in-house R&D centerwith complete supporting facilities guarantees the high quality of products.
There is no MOQ request for all our products!
Certifications
Product Process
WHY YOU CHOOSE ROHI.
1,We have experience of manufacture for various rapid test kit.
2.Our customers are all over the world ,so you will get the most professional service.
3.We are the golden supplier of Alibaba, and we are the long-term top supplier of globalsources.
4.We have lots of Rapid test series for you reference and choosing.
5.If you have any request,email us,you will get the quickest reply.
