Rohi Milk Antibiotic residues test kit to Streptomycin

Streptomycin Rapid Test (Milk)

1.        INTENDED USE

Rohi Streptomycin Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Streptomycin residue in milk.

Cut-off:   150 ppb

Assay Time:  5 – 10 min

Sample: Defatted milk

2.        PRINCIPLE OF THE ASSAY

Rohi Streptomycin Rapid Test is based on competitive lateral flow immunochromatographic assay. The Streptomycin conjugate in the test zone will capture the immuno-gold (colloid gold- Streptomycin antibody conjugate), when there is very little dissociative Streptomycin in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Streptomycin is present in concentration of 100 ppb and above which explains a positive result.

3.        KIT COMPONENT

-           10×foil pouches each contain one cassette and a desiccant

-           10×centrifugal tubes (1.5 mL)

-           Product Manual

4.        TEST PROCEDURE

-           Bring 2-3 mL of the under tested milk to room temperature (20-30℃).

-           Collect 1mL of milk sample into a centrifugal tube. Do centrifugation at 7000 rpm for 3-4 min to separate the fat component.

-           Take out the cassette from the foil pouch and place it horizontally.

-           Insert the pipette through the fat layer (5 mm below) to collect the defatted milk and gradually drip 3 drops into the assay sample hole “S”.

-           Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid

5.        INTERPRETATION OF RESULTS

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the

concentration of Streptomycin is at or above 150 ppb in the samples.

Negative: The presence of both clear band in C zone and T zone.

Invalid: No colored band appears in C zone.

6.        SPECIFICITY

The results are negative when the test card is applied to detect 100 ppm of Chloramphenicol, Macrolides, Neomycin, Gentamicin, Tetracycline and Beta-lactam.

7.        STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8.        PRECAUTIONS

-           For best results, please strictly adhere to these instructions.

-           All reagents must be at room temperature before running the assay.

-           Do not remove test cassette from its pouch until immediately before use.

-           Do not reuse the test kit.

-           Do not use the test beyond its expiration date marked on the foil pouch.

-           The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

9.        LIMITATION

Rohi Streptomycin Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Streptomycin above 150 ppb in milk. If you want a quantitative result, it is suggested to apply other method such as ELISA / HPLC in practice.