TransGen Biotech pEASY Blunt E1 Expression Kit Expression Plasmid Vector
TransGen Biotech pEASY-Blunt E1 Expression Kit-Expression Plasmid Vector
TransGen Biotech pEASY-Blunt E1 Expression Kit-Expression Plasmid Vector
TransGen Biotech pEASY-Blunt E1 Expression Kit-Expression Plasmid Vector

TransGen Biotech pEASY Blunt E1 Expression Kit Expression Plasmid Vector

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Price:RUB 4,590.62 - RUB 5,355.72

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Product Overview

Description

pEASY-Blunt E1 Expression Kit

pEASY®- Blunt E2 Expression Vector is constructed from pET vector, it utilizes a highly efficient, five-minute blunt cloning strategy to clone a PCR product into a high-efficient expression vector under tight regulation of T7lac promoter. The size of control insert is 750 bp, and expressed target protein is about 27 kDa.

Cat. No. CE211
Specification 10 rxns
Storage  Trans1-T1 Phage Resistant Chemically Competent Cells at -70oC for six months
Others at -20oC for nine months
Description • 5 minutes fast ligation of Pfu-amplified PCR products.
• Ampicillin resistance.

• T7 promoter primer and T7 terminator primer for sequencing.

• Bacteriophage T7lac promoter for high level expression.
• N-terminal 6 × His tag for easy purification.

• Trans1-T1 phage resistant chemically competent cell, high  

  transformation efficiency (>109 cfu/μg pUC19 DNA) and fast

  growing.

 

Kit Contents

Component

CE211-01

(10 rxns)

 

pEASY®- Blunt E2 Expression Vector (15 ng/μl)

10 μl

EControl Template (5 ng/μl)

10 μl

EControl Forward Primers (10 μM)

10 μl

EControl Reverse Primers (10 μM)

10 μl

T7 Promoter Primer (10 μM)

50 μl

T7 Terminator Primer (10 μM)

50 μl

Trans1-T1 Phage Resistant

Chemically Competent Cell

 5×100 μl

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Packaging

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