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Phosphatase Inhibitor
ProteinSafeTM Phosphatase Inhibitor Cocktail, EDTA-free (100×) is a ready-to-use mixture of six protease inhibitors (AEBSF,Aprotinin, Bestatin, E-64, Leupeptin, and Pepstatin A)that has been optimized to protect proteins from being digested by endogenous proteases, including serine protease, cysteine protease, aminopeptidase and aspartic acid protease. This cocktail is suitable for use in protein purification, Western Blot, Co-IP, ChIP, protein kinase activity assay, etc.The cocktail does not contain EDTA (ametalloprotease inhibitor), which is incompatible with some downstream applications (i.e. protein assays, 2D electrophoresis, etc.).
| Cat. No. | DI101 | |
| Specification | DI101-01 | 500ul |
| DI101-02 | 1ml | |
| Concentration: | 50 units/μl | |
| Storage: | at -20°C for one year | |
| Component: | DI101-01 | DI101-02 |
| ProteinSafeTM Phosphatase Inhibitor Cocktail , EDTA-free (100×): | 500 μl | 1 ml |
NOTE :
This cocktail is generally effective when used at a 1× final concentration. However, samples with highlevels of proteases might
require a more concentrated treatment (i.e., 2-3×).
• If the inhibition of phosphatases is required, please use ProteinSafeTM Phosphatase Inhibitor Cocktail (DI201) along with this
cocktail.
• The cocktail should be stored at -20°C for long term storage or at 2-8°C if used within a short time.


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