Glucoamylase PUREMEI PG is made from NON-GMO strains of Aspergillus niger through submerged fermentation, extraction and refining processes. PUREMEI PG has a high conversion rate of starchy substrates into fermentable sugars. It can hydrolyze α-D-1, 4 glucosidic bonds from the non-reducing end of starch one after. It can also hydrolyze the α-D-1, 6 glucoside branch bonds of starch and cleaves the α-1, 3 bonds to release glucose.
PUREMEI PG is designed with a robust glucoamylase activity for saccharification in fuel ethanol production. Use of PUREMEI PG provides higher ethanol yields, faster fermentations, and lower residual starch levels.
EC 220.127.116.11, 1,4-alpha-D-glucan glucohydrolase
130,000 U/ml (minimum)
3.0 to 5.0
1.10 to 1.20 g/ml
EFFECT OF PH
The optimal pH for PUREMEI PG is 4.0 to 4.5. See Figure 1.
EFFECT OF TEMP
The effective temperature range for PUREMEI PG is 30 to 65°C. The optimal temperature range is 55 to 60°C. See Figure 2.
FIGURE 1: EFFECT OF PH ON ACTIVITY FIGURE 2: EFFECT OF TEMP ON ACTIVITY
PUREMEI PG is generally added at a level of 0.065 to 0.085% w/w (dry solids basis). The actual dosage depends on the actual processing conditions including raw materials, temperature, reaction time, pH and the desired results in individual ethanol plants.
This enzyme has been formulated for optimal stability. Typical shelf-life is six months provided it is stored below 25°C in its original packaging, sealed and unopened, protected from the sunlight. Its shelf-life will be eighteen months if it is stored properly at 0～10°C. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.
SAFETY & HANDLING
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to Material Safety Data Sheet.