Quick Bacteria Genomic DNA Extraction Kit/ DNA Isolation Kit N1151/N1152
- Category: Specific Reagents >>>
- Supplier: Guangzhou Dongsheng Biotech Co. Ltd. [Guangdong China]Guangzhou
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Product Overview
Description
Quick Bacteria Genomic DNA Ectraction Kit
For research use only
Kit Content
Content | N1151 | N1152 |
Solution DS | 15 ml | 30 ml |
Solution MS | 20 ml | 40 ml |
Proteinase K | 1 ml | 2 ml |
Wash Buffer PS | 30 ml | 60 ml |
Wash Buffer PE | 15 ml | 30 ml |
Eluent Buffer TE | 5 ml | 10 ml |
Spin Column | 50 each | 100 each |
Description
The bacteria Genomic DNA Purification Kit provides a simple and rapid method for high quality genomic DNA purification from Gram Positive and Gram Negative Bacteria. The bacteria Genomic DNA system uses the silica-gel-membrane technology for simple and fast isolation of Genomic DNA without phenol/chloroform. Homogenization is not necessary since tissues are directly lysed by Proteinase K. The buffer system is optimized to allow selective binding of DNA to the silica-gel membrane. The simple centrifugation protocol completely removes contaminants such as proteins, divalent cations, and secondary metabolites. Pure DNA is then eluted in water or low-salt buffer, ready to use. The typical yield of genomic DNA is 3-20 µg from 0.5 – 2 ml of bacteria culure. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis.
Applications
Purified DNA is free from contaminants and enzyme inhibitors, and typically has A260/A280 ratios between 1.7 and 1.9, and is suitable for applications such as:
- Restriction digestion
- PCR
- Labeling
- Library construction
Feature
- Safe - no phenol extraction step.
- Fast - procedure takes only 30 min.
- Efficient – 3-20 µg of genomic DNA from 1.5 – 2 ml bacteria culure.
- High purity - Purified DNA is ready for downstream application such as PCR, restriction digestion.
Store
store Protein K at -20°C, other reagents can be store at room temperature for up to 1 year. Any precipitate in the Solution DS and solution MS can be re-dissolved by incubating at 37°C before use.
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